Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer
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Xi Xiao1,*, Carsten Sticht2,*, Libo Yin1, Li Liu1, Svetlana Karakhanova1, Yefeng Yin1, Christina Georgikou1, Jury Gladkich1, Wolfgang Gross1, Norbert Gretz2,# and Ingrid Herr1,#
1 Molecular OncoSurgery Group, Section of Surgical Research, Department of General, Visceral and Transplant Surgery, University of Heidelberg, Heidelberg, Germany
2 Medical Research Centre, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany
* These authors contributed equally to this work and share the first authorship
# These authors contributed equally to this work and share the last authorship
|Ingrid Herr,||email:||[email protected]e|
|Norbert Gretz,||email:||[email protected]|
Keywords: broccoli; broccoletti; Brassica rapa sylvestris; plant microRNAs; cross-kingdom regulation
Abbreviations: miRs: microRNAs; PDA: pancreatic ductal adenocarcinoma
Received: February 05, 2020 Accepted: March 03, 2020 Published: April 07, 2020
Food-derived plant microRNAs are suggested to control human genes by “cross-kingdom” regulation. We examined microRNAs in sprouts from Brassica rapa sylvestris, known as broccoletti, which are widely used as sulforaphane supplements, and assessed their influence on pancreatic cancer. RNA was isolated from 4-day-old sprouts, followed by deep sequencing and bioinformatic analysis. We identified 2 new and 745 known plant microRNA sequences in the miRbase database and predicted 15,494 human target genes and 76,747 putative 3′-UTR binding sites in these target genes. The most promising candidates were the already known microRNA sequence bra-miR156g-5p and the new sequence Myseq-330, both with predicted human target genes related to apoptosis. The overexpression of the respective oligonucleotides by lipofection did not alter the viability, apoptosis, clonogenicity, migration or associated protein expression patterns in pancreatic cancer cells. These data demonstrate that broccoletti sprouts contain microRNA sequences with putative binding sites in human genes, but the sequences evaluated here did not affect cancer growth. Our database of broccoletti-derived microRNA sequences provides a valuable tool for future analysis.
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