Oncotarget

Research Papers:

Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosomes has antineoplastic effects for gallbladder cancer

Hidehiko Hishikawa, Masaki Kaibori _, Takumi Tsuda, Kosuke Matsui, Tadayoshi Okumura, Eiichi Ozeki and Kengo Yoshii

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Oncotarget. 2019; 10:5622-5631. https://doi.org/10.18632/oncotarget.27193

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Abstract

Hidehiko Hishikawa1, Masaki Kaibori1, Takumi Tsuda1, Kosuke Matsui1, Tadayoshi Okumura1, Eiichi Ozeki2 and Kengo Yoshii3

1 Department of Surgery, Kansai Medical University, Hirakata, Osaka, Japan

2 Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan

3 Department of Mathematics and Statistics in Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan

Correspondence to:

Masaki Kaibori,email: Kaibori@hirakata.kmu.ac.jp

Keywords: near infrared fluorescence imaging; photodynamic therapy; indocyanine green; lactosome; gall bladder cancer

Received: May 28, 2019     Accepted: August 16, 2019     Published: September 24, 2019

ABSTRACT

BACKGROUND: The diagnostic use and therapeutic effect of near infrared fluorescence (NIF) imaging and photodynamic therapy (PDT) was investigated for gallbladder cancer using indocyanine green (ICG)-lactosomes.

RESULTS: PDT was toxic for NOZ cells treated with ICG-lactosomes. Fluorescence intensity in the tumor region of mice administered ICG-lactosomes, but not ICG alone, was higher than the healthy contralateral region ≥24 hours after injection. PDT exerted immediate and continuous phototoxic effects in NOZ implanted mice injected with ICG-lactosomes. Enhanced antitumor effects were observed in the twice irradiated group compared with the once irradiated group.

METHOD: ICG or ICG-lactosomes were added to the human gallbladder cancer cell line NOZ followed by PDT and cell viability was measured. Mass spectrometry of ICG and ICG-lactosomes was performed after PDT. ICG or ICG-lactosomes were intravenously administered to BALB/c nude mice implanted subcutaneously with NOZ cells and fluorescence was evaluated by NIF imaging. Implanted tumors underwent PDT and antitumor effects were analyzed after performing irradiation once or twice in ICG-lactosome groups.

CONCLUSIONS: ICG-lactosomes accumulated in xenograft tumors and PDT had an antitumor effect on these malignant tumors. NIF imaging with ICG-lactosomes and PDT may be useful diagnostic and/or therapeutic agents for gallbladder cancer.


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