Research Papers:

A three-step approach identifies novel shear stress-sensitive endothelial microRNAs involved in vasculoprotective effects of high-intensity interval training (HIIT)

Boris Schmitz, Franziska L. Breulmann, Bothaynah Jubran, Florian Rolfes, Lothar Thorwesten, Michael Krüger, Andreas Klose, Hans-Joachim Schnittler and Stefan-Martin Brand

PDF  |  Full Text  |  Supplementary Files  |  How to cite

Oncotarget. 2019; 10:3625-3640. https://doi.org/10.18632/oncotarget.26944

Metrics: PDF 1120 views  |   Full Text 2299 views  |   ?  


Boris Schmitz1,*, Franziska L. Breulmann1,*, Bothaynah Jubran2, Florian Rolfes1, Lothar Thorwesten1, Michael Krüger3, Andreas Klose3, Hans-Joachim Schnittler2 and Stefan-Martin Brand1

1 Institute of Sports Medicine, Molecular Genetics of Cardiovascular Disease, University Hospital Muenster, Muenster, Germany

2 Institute of Anatomy and Vascular Biology, University of Muenster, Muenster, Germany

3 Department of Physical Education and Sports History, University of Muenster, Muenster, Germany

* These authors contributed equally to this work

Correspondence to:

Boris Schmitz,email: [email protected]

Keywords: miRNA; shear stress; performance; endothelium; high-intensity training (HIT)

Received: November 17, 2018     Accepted: April 03, 2019     Published: June 04, 2019


Circulatory microRNAs (c-miRNAs) are regulated in response to physical activity and may exert anti-atherosclerotic effects. Since the vascular endothelium is an abundant source of c-miRNAs, we aimed to identify novel vasculoprotective exercise-induced c-miRNAs by the combined analysis of published endothelial miRNA array data followed by in vivo and in vitro validation. We identified 8 different array-based publications reporting 185 endothelial shear stress-regulated miRNAs of which 13 were identified in ≥3 independent reports. Nine miRNAs had already been associated with physical activity. Of the remaining novel miRNAs, miR-98-3p and miR-125-5p were selected for further analysis due to reported vasculoprotective effects. Analysis in two different 4-week high-intensity interval training (HIIT) groups (group 1 [n=27]: 4x30 s, group 2 [n=25]: 8x15 s; all-out running) suggested significantly elevated miR-98 and miR-125a-5p levels in response to acute exercise at baseline and at follow-up. Endothelial in vitro shear stress experiments revealed increased miR-125a-5p and miR-98-3p levels in medium of human umbilical vein endothelial cells at 30 dyn/cm2 after 20 and 60 min, respectively. Our results suggest that miR-98-3p and miR-125a-5p can be rapidly secreted by endothelial cells, which might be the source of increased c-miR-98-3p and -125a-5p levels in response to HIIT. Both miRNAs attenuate endothelial inflammation and may mediate vasculoprotective effects of physical exercise including HIIT.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 26944