Oncotarget

Research Papers:

Carbonic anhydrase 9 expression is associated with poor prognosis, tumor proliferation, and radiosensitivity of thymic carcinomas

Yoichi Ohtaki, Kimihiro Shimizu _, Reika Kawabata-Iwakawa, Navchaa Gombodorj, Bolag Altan, Susumu Rokudai, Arito Yamane, Kyoichi Kaira, Takehiko Yokobori, Toshiteru Nagashima, Kai Obayashi, Seshiru Nakazawa, Misaki Iijima, Takayuki Kosaka, Toshiki Yajima, Akira Mogi, Hiroyuki Kuwano, Ken Shirabe and Masahiko Nishiyama

PDF  |  Full Text  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2019; 10:1306-1319. https://doi.org/10.18632/oncotarget.26657

Metrics: PDF 523 views  |   Full Text 785 views  |   ?  


Abstract

Yoichi Ohtaki1,2,3, Kimihiro Shimizu1,2, Reika Kawabata-Iwakawa4, Navchaa Gombodorj2, Bolag Altan5, Susumu Rokudai4, Arito Yamane4, Kyoichi Kaira5, Takehiko Yokobori6, Toshiteru Nagashima1,2, Kai Obayashi1,2, Seshiru Nakazawa1,2, Misaki Iijima1,2, Takayuki Kosaka1,2, Toshiki Yajima1,2, Akira Mogi1,2, Hiroyuki Kuwano2, Ken Shirabe2 and Masahiko Nishiyama3,4

1Division of General Thoracic Surgery, Integrative Center of General Surgery, Gunma University Hospital, Maebashi, Gunma, Japan

2Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan

3Education and Research Support Center, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan

4Department of Molecular Pharmacology and Oncology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan

5Department of Oncology Clinical Development, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan

6Department of Innovative Cancer Immunotherapy, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan

Correspondence to:

Kimihiro Shimizu, email: kmshimizu@gmail.com

Keywords: thymic epithelial tumor; thymoma; thymic carcinoma; CA9; hypoxia

Received: May 25, 2018     Accepted: January 22, 2019     Published: February 12, 2019

ABSTRACT

Introduction: Thymic epithelial tumors (TETs) comprise several histologies of thymoma and thymic carcinomas (TCs), and TC frequently metastasizes and causes death. We therefore aimed here to identify key molecules closely related to prognosis and their biological roles in high-risk TETs, particularly TCs.

Results: RNA sequence analysis demonstrated that hypoxia-related genes were highly expressed in TETs. The expression of the hypoxia-related gene CA9 was noteworthy, particularly in TCs. Immunohistochemical analysis revealed that CA9 was expressed in 81.0% of TCs and 20.7% of all TET samples. CA9 expression was significantly associated with Masaoka stage, WHO classification, and recurrence-free survival after tumor resection (P = 0.005). The down-regulation of CA9 transcription in TC cell lines by small interfering RNAs significantly inhibited CA9 expression, which inhibited proliferation and increased sensitivity to irradiation.

Conclusions: CA9 expression may serve as a significant prognostic marker of TETs and therefore represents a potential target for the development of novel drugs and radiation-sensitizing therapy designed to improve the outcomes of patients with TCs.

Materials and Methods: We performed comprehensive transcriptome sequencing of 23 TETs and physiologic thymic specimens to identify genes highly and specifically expressed in high-risk TETs, particulary TCs. We performed immunohistochemical analysis of 179 consecutive surgically resected TETs to evaluate the significance of the association of protein expression with clinicopathological features and prognosis. The biological significance of the most promising prognostic marker was further studied using the TC cell lines, Ty-82 and MP57.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 26657