PRK1/PKN1 controls migration and metastasis of androgen-independent prostate cancer cells
Metrics: PDF 2104 views | HTML 3750 views | ?
Cordula A. Jilg1, Anett Ketscher1,2, Eric Metzger1, Barbara Hummel1, Dominica Willmann1, Vanessa Rüsseler1,3, Vanessa Drendel4, Axel Imhof5, Manfred Jung6, Henriette Franz1, Stefanie Hölz1,2, Malte Krönig1, Judith M. Müller1, Roland Schüle1,7,8
1Urologische Klinik und Zentrale Klinische Forschung, Klinikum der Universität Freiburg, Freiburg 79106, Germany
2University of Freiburg, Faculty of Biology, Freiburg 79104, Germany
3Universitätsklinikum Köln, Institut für Pathologie, Köln 50937, Germany
4Department of Pathology, University Medical Center, Freiburg, Germany
5Adolf-Butenandt Institute and Munich Center of Integrated Protein Science (CIPS), Ludwig-Maximilians-University of Munich, Munich 80336, Germany
6Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg 79104, Germany
7BIOSS Centre of Biological Signalling Studies, Albert-Ludwigs-University, Freiburg, Germany
8Deutsches Konsortium für Translationale Krebsforschung (DKTK), Standort Freiburg, Germany
R. Schüle, e-mail: firstname.lastname@example.org
Received: August 06, 2014 Accepted: October 26, 2014 Published: December 10, 2014
The major threat in prostate cancer is the occurrence of metastases in androgen-independent tumor stage, for which no causative cure is available. Here we show that metastatic behavior of androgen-independent prostate tumor cells requires the protein-kinase-C-related kinase (PRK1/PKN1) in vitro and in vivo. PRK1 regulates cell migration and gene expression through its kinase activity, but does not affect cell proliferation. Transcriptome and interactome analyses uncover that PRK1 regulates expression of migration-relevant genes by interacting with the scaffold protein sperm-associated antigen 9 (SPAG9/JIP4). SPAG9 and PRK1 colocalize in human cancer tissue and are required for p38-phosphorylation and cell migration. Accordingly, depletion of either ETS domain-containing protein Elk-1 (ELK1), an effector of p38-signalling or p38 depletion hinders cell migration and changes expression of migration-relevant genes as observed upon PRK1-depletion. Importantly, a PRK1 inhibitor prevents metastases in mice, showing that the PRK1-pathway is a promising target to hamper prostate cancer metastases in vivo.
Statement of significance
Here we describe a novel mechanism controlling the metastatic behavior of PCa cells and identify PRK1 as a promising therapeutic target to treat androgen-independent metastatic prostate cancer.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.