Oncotarget

Clinical Research Papers:

Prognostic impact of a compartment-specific angiogenic marker profile in patients with pancreatic cancer

Christoph Kahlert _, Maria Fiala, Gabriel Musso, Niels Halama, Sophia Keim, Massimiliano Mazzone, Felix Lasitschka, Mathieu Pecqueux, Fee Klupp, Thomas Schmidt, Nuh Rahbari, Sebastian Schölch, Christian Pilarsky, Alexis Ulrich, Martin Schneider, Juergen Weitz and Moritz Koch

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2014; 5:12978-12989. https://doi.org/10.18632/oncotarget.2651

Metrics: PDF 1537 views  |   HTML 2058 views  |   ?  


Abstract

Christoph Kahlert1,*, Maria Fiala1,*, Gabriel Musso2,3, Niels Halama4, Sophia Keim4, Massimiliano Mazzone5,6, Felix Lasitschka7, Mathieu Pecqueux8, Fee Klupp1, Thomas Schmidt1, Nuh Rahbari8, Sebastian Schölch8, Christian Pilarsky8, Alexis Ulrich1, Martin Schneider1, Juergen Weitz8, Moritz Koch8

1Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg 69120, Germany

2Department of Medicine, Harvard Medical School, Boston, MA 02115, USA

3Cardiovascular Division, Brigham and Women’s Hospital, Boston, MA 02115, USA

4Medical Oncology, National Center for Tumor Diseases and Hamamatsu Tissue Imaging and Analysis (TIGA) Center, Institute for Medical Biometry and Informatics, University of Heidelberg, Germany

5Laboratory of Molecular Oncology and Angiogenesis, Vesalius Research Center, VIB, Leuven 3000, Belgium

6Laboratory of Molecular Oncology and Angiogenesis, Vesalius Research Center, Department of Oncology, KU, Leuven 3000, Belgium

7Institute of Pathology, University of Heidelberg, Heidelberg 69120, Germany

8Department of General, Visceral and Thoracic Surgery, University of Dresden, Dresden 01307, Germany

*These authors contributed equally to this work

Correspondence to:

Christoph Kahlert, e-mail: christoph.kahlert.079@googlemail.com

Keywords: Pancreatic cancer, stroma, angiogenic cytokines, expression profile

Received: August 05, 2014     Accepted: October 27, 2014     Published: December 30, 2014

ABSTRACT

Pancreatic cancer consists of a heterogenous bulk of tumor cells and stroma cells which contribute to tumor progression by releasing angiogenic factors. Those factors can be detected as circulating serum factors. We performed a compartment-specific analysis of tumor-derived and stroma-derived angiogenic factors to identify biomarkers and molecular targets for the treatment of pancreatic cancer. Kryo-frozen tissue from primary ductal adenocarcinomas (n = 51) was laser-microdissected to isolate tumor and stroma tissue. Expression of 17 angiogenic factors (angiopoietin-2, follistatin, GCSF, HGF, interleukin-8, leptin, PDGF-BB, PECAM-1, VEGF, matrix metalloproteinase -1, -2, -3, -7, -9, -10, -12, and -13) was analyzed using a multiplex elisa assay for tissue-derived proteins and corresponding serum.

Our study reveals a compartment-specific expression profile for several angiogenic factors and matrix metalloproteinases. ROC analysis of corresponding serum samples reveals MMP-7 and MMP-12 as strong classifiers for the diagnosis of patients with pancreatic cancer vs. healthy control donors. High expression of tumor-derived PDGF-BB and MMP-1 correlates with prolonged survival in univariate and multivariate analysis. In conclusion, a distinct expression patterns for angiogenic cytokines and MMPs in pancreatic cancer and surrounding stroma may implicate them as novel targets for cancer treatment. Tumor-derived PDGF-BB and MMP-1 are significant and independent prognostic markers for poor survival.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 2651