Research Papers:

This article has been corrected. Correction in: Oncotarget. 2018; 9:33050.

Prosopis juliflora (Sw.), DC induces apoptosis and cell cycle arrest in triple negative breast cancer cells: in vitro and in vivo investigations

Bhimashankar Gurushidhappa Utage, Milind Shivajirao Patole, Punam Vasudeo Nagvenkar, Sonali Shankar Kamble and Rajesh Nivarti Gacche _

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Oncotarget. 2018; 9:30304-30323. https://doi.org/10.18632/oncotarget.25717

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Bhimashankar Gurushidhappa Utage1,2, Milind Shivajirao Patole1, Punam Vasudeo Nagvenkar1, Sonali Shankar Kamble2 and Rajesh Nivarti Gacche2,3

1National Centre for Cell Science, NCCS Complex, Pune, 411007, MS, India

2School of Life Sciences, S.R.T.M. University, Nanded, 4316069, MS, India

3Department of Biotechnology, Savitribai Phule Pune University, Pune, 411007, MS, India

Correspondence to:

Rajesh Nivarti Gacche, email: [email protected]

Keywords: P. juliflora; triple negative breast cancer; tumor inhibition; apoptosis; ROS

Received: February 09, 2018    Accepted: June 13, 2018    Published: July 13, 2018


Plant originated drugs/formulations are extensively prescribed by the physicians as a complementary therapy for treating various human ailments including cancer. In this study Prosopis juliflora leaves methanol extract was prepared and exposed to human breast cancer cell lines i.e. MDA-MB-231 and MCF-7 and human keratinocytes HaCaT as a representative of normal cells. Initially, a series of in vitro experiments like cell proliferation, migration, colony formation, cell cycle arrest and inhibition of angiogenesis. After confirmation of the efficient and selective activity against triple negative breast cancer cell line, we further evaluated the possible mechanism of inducing cell death and experiments like detection of reactive oxygen species, caspases and poly (ADP-ribose) polymerase cleavage study and Annexin V assay were performed. We also evaluated in vivo anti tumorigenic activity of the P. juliflora leaves by using 4T1 cells (a triple negative mouse origin breast cancer cell line) and BALB/c xenograft mouse model. In vitro experiments revealed that methanol extract of Prosopis juliflora leaves possess impressive anti-breast cancer activity more specifically against triple negative breast cancer cells, while the in vivo studies demonstrated that P. juliflora leaves extract significantly suppressed the 4T1 induced tumor growth. Present investigations clearly focus the significance of P. juliflora as an important resource for finding novel leads against triple negative breast cancer. The results may also act as a ready reference towards developing P. juliflora based formulation as an alternative and complementary medicine for the management of breast cancer.

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