Research Papers:

The proteoglycan-like domain of carbonic anhydrase IX mediates non-catalytic facilitation of lactate transport in cancer cells

Samantha Ames, Silvia Pastorekova and Holger M. Becker _

PDF  |  HTML  |  How to cite

Oncotarget. 2018; 9:27940-27957. https://doi.org/10.18632/oncotarget.25371

Metrics: PDF 1486 views  |   HTML 3225 views  |   ?  


Samantha Ames1, Silvia Pastorekova2 and Holger M. Becker1,3

1Division of General Zoology, Department of Biology, University of Kaiserslautern, Kaiserslautern, Germany

2Department of Tumor Biology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak Republic

3Institute of Physiological Chemistry, University of Veterinary Medicine Hannover, Hannover, Germany

Correspondence to:

Holger M. Becker, email: [email protected]

Keywords: monocarboxylate transporter; proton antenna; hypoxia; breast cancer; Xenopus oocyte

Received: November 29, 2017     Accepted: April 19, 2018     Published: June 15, 2018


Highly glycolytic tumor cells release vast amounts of lactate and protons via monocarboxylate transporters (MCTs), which exacerbate extracellular acidification and support the formation of a hostile environment. Transport activity of MCTs can be facilitated by non-catalytic interaction with carbonic anhydrase IX (CAIX), the expression of which has been shown to be upregulated under hypoxia. We have now studied the mechanisms that enable CAIX-mediated facilitation of proton-coupled lactate transport in breast cancer cells and Xenopus oocytes. Our results indicate that the proteoglycan like (PG) domain of CAIX could function as ‘proton antenna’ to facilitate MCT transport activity. Truncation of the PG domain and application of a PG-binding antibody significantly reduced proton-coupled lactate transport in MCT-expressing oocytes and hypoxic breast cancer cells, respectively. Furthermore, application of the PG-binding antibody reduced proliferation and migration of hypoxic cancer cells, suggesting that facilitation of proton-coupled lactate flux by the CAIX PG domain contributes to cancer cell survival under hypoxic conditions.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 25371