Hemidesmus indicus induces immunogenic death in human colorectal cancer cells
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Eleonora Turrini1,*, Elena Catanzaro1,*, Manuele G. Muraro2,*, Valeria Governa3, Emanuele Trella2, Valentina Mele3, Cinzia Calcabrini1, Fabiana Morroni4, Giulia Sita4, Patrizia Hrelia4, Massimo Tacchini5 and Carmela Fimognari1
1Department for Life Quality Studies, Alma Mater Studiorum–University of Bologna, Rimini, Italy
2Oncology Surgery, Department of Biomedicine, University Hospital of Basel and University of Basel, ZLF, Basel-Switzerland
3Cancer Immunotherapy, Department of Biomedicine, University Hospital of Basel and University of Basel, ZLF, Basel-Switzerland
4Department of Pharmacy and Biotechnology, Alma Mater Studiorum–University of Bologna, Bologna, Italy
5Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy
*These authors contributed equally to this work
Carmela Fimognari, email: firstname.lastname@example.org
Keywords: Hemidesmus indicus; immunogenic cell death; adjuvant activity; botanical drugs; colorectal cancer
Received: July 29, 2017 Accepted: April 16, 2018 Published: May 11, 2018
The ability of anticancer treatments to promote the activation of tumor-reactive adaptive immune responses is emerging as a critical requirement underlying their clinical effectiveness. We investigated the ability of Hemidesmus indicus, a promising anticancer botanical drug, to stimulate immunogenic cell death in a human colorectal cancer cell line (DLD1). Here we show that Hemidesmus treatment induces tumor cell cytotoxicity characterized by surface expression of calreticulin, increased HSP70 expression and release of ATP and HMGB1. Remarkably, the exposure to released ICD-inducer factors from Hemidesmus-treated DLD1 cells caused a modest induction of CD14-derived dendritic cells maturation, as demonstrated by the increased expression of CD83. Moreover, at sub-toxic concentrations, H.i. treatment of monocytes and dendritic cells induced their mild activation, suggesting its additional direct immunostimulatory activity. These data indicate that Hemidesmus indicus induces immunogenic cell death in human tumor cells and suggest its potential relevance in innovative cancer immunotherapy protocols.
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