Research Papers:

The isoform A of reticulon-4 (Nogo-A) in cerebrospinal fluid of primary brain tumor patients: influencing factors

Olga Martyna Koper _, Joanna Kamińska, Anna Milewska, Karol Sawicki, Zenon Mariak, Halina Kemona and Joanna Matowicka-Karna

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Oncotarget. 2018; 9:25048-25056. https://doi.org/10.18632/oncotarget.25278

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Olga Martyna Koper1, Joanna Kamińska1, Anna Milewska2, Karol Sawicki3, Zenon Mariak3, Halina Kemona1 and Joanna Matowicka-Karna1

1Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland

2Department of Statistics and Medical Informatics, Medical University of Bialystok, Bialystok, Poland

3Department of Neurosurgery, Clinical Hospital of the Medical University of Bialystok, Bialystok, Poland

Correspondence to:

Olga Martyna Koper, email: [email protected]

Joanna Matowicka-Karna, email: [email protected]

Keywords: cerebrospinal fluid; isoform A of reticulon-4 (Nogo-A); primary brain tumor

Received: August 24, 2017     Accepted: April 06, 2018     Published: May 18, 2018


Background: The influence of isoform A of reticulon-4 (Nogo-A), also known as neurite outgrowth inhibitor, on primary brain tumor development was reported. Therefore the aim was the evaluation of Nogo-A concentrations in cerebrospinal fluid (CSF) and serum of brain tumor patients compared with non-tumoral individuals.

Results: All serum results, except for two cases, obtained both in brain tumors and non-tumoral individuals, were below the lower limit of ELISA detection. Cerebrospinal fluid Nogo-A concentrations were significantly lower in primary brain tumor patients compared to non-tumoral individuals. The univariate linear regression analysis found that if white blood cell count increases by 1 × 103/μL, the mean cerebrospinal fluid Nogo-A concentration value decreases 1.12 times. In the model of multiple linear regression analysis predictor variables influencing cerebrospinal fluid Nogo-A concentrations included: diagnosis, sex, and sodium level. The mean cerebrospinal fluid Nogo-A concentration value was 1.9 times higher for women in comparison to men. In the astrocytic brain tumor group higher sodium level occurs with lower cerebrospinal fluid Nogo-A concentrations. We found the opposite situation in non-tumoral individuals.

Conclusions: Univariate linear regression analysis revealed, that cerebrospinal fluid Nogo-A concentrations change in relation to white blood cell count. In the created model of multiple linear regression analysis we found, that within predictor variables influencing CSF Nogo-A concentrations were diagnosis, sex, and sodium level. Results may be relevant to the search for cerebrospinal fluid biomarkers and potential therapeutic targets in primary brain tumor patients.

Materials and methods: Nogo-A concentrations were tested by means of enzyme-linked immunosorbent assay (ELISA).

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