Stability and anti-tumor effect of oncolytic herpes simplex virus type 2
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Yang Wang1,*, Jing Jin1,*, Zhen Wu1,*, Sheng Hu2,*, Han Hu1, Zhifeng Ning3, Yanfei Li4, Yuting Dong5, Jianwen Zou4, Zeyong Mao5, Xiaotai Shi5, Huajun Zheng6, Shuang Dong2, Fuxing Liu3, Zhizheng Fang5, Jiliang Wu7 and Binlei Liu1,4
1National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, Hubei, China
2Department of Medical Oncology, Hubei Cancer Hospital, Wuhan, 430079, Hubei, China
3Basic Medicine College, Hubei University of Science and Technology, Xianning, 437100, Hubei, China
4College of Pharmacology, Hubei University of Science and Technology, Xianning, 437100, Hubei, China
5Wuhan Binhui Biotechnology Co., Ltd., Wuhan, 430075, Hubei, China
6Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, 201203, Shanghai, China
7Hubei Provincial Key Laboratory of Cardiocerebrovascular and Metabolic Diseases, Hubei University of Science and Technology, Xianning, 437100, Hubei, China
*These authors have contributed equally to this work
Binlei Liu, email: email@example.com
Jiliang Wu, email: firstname.lastname@example.org
Keywords: herpes simplex virus type 2; stability; anti-tumor effect
Received: August 24, 2017 Accepted: March 22, 2018 Published: May 15, 2018
Oncolytic virotherapy is a new therapeutic strategy based on the inherent cytotoxicity of viruses and their ability to replicate and spread in tumors in a selective manner. We constructed a new type of oncolytic herpes simplex virus type 2 (oHSV-2, named OH2) to treat human cancers, but a systematic evaluation of the stability and oncolytic ability of this virus is lacking. In this study, we evaluated its physical stability, gene modification stability and biological characteristics stability, including its anti-tumor activity in an animal model. The physical characteristics as well as genetic deletions and insertions in OH2 were stable, and the anti-tumor activity remained stable even after passage of the virus for more than 20 generations. In conclusion, OH2 is a virus that has stable structural and biological traits. Furthermore, OH2 is a potent oncolytic agent against tumor cells.
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