Atomistic molecular dynamics simulations of bioactive engrailed 1 interference peptides (EN1-iPeps)
Metrics: PDF 1302 views | HTML 1842 views | ?
Neha S. Gandhi1, Pilar Blancafort2 and Ricardo L. Mancera3
1School of Mathematical Sciences and Institute for Health and Biomedical Innovation, Queensland University of Technology, Gardens Point Campus, Brisbane QLD 4000, Australia
2Cancer Epigenetics Group, The Harry Perkins Institute of Medical Research, Perth WA 6009, Australia
3School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute and Curtin Institute for Computation, Curtin University, Perth WA 6845, Australia
Neha S. Gandhi, email: [email protected]
Ricardo L. Mancera, email: [email protected]
Keywords: Engrailed homeodomain; transcription factor; iPep; breast cancer; molecular dynamics
Received: July 27, 2017 Accepted: March 15, 2018 Published: April 27, 2018
The neural-specific transcription factor Engrailed 1 - is overexpressed in basal-like breast tumours. Synthetic interference peptides - comprising a cell-penetrating peptide/nuclear localisation sequence and the Engrailed 1-specific sequence from the N-terminus have been engineered to produce a strong apoptotic response in tumour cells overexpressing EN1, with no toxicity to normal or non Engrailed 1-expressing cells. Here scaled molecular dynamics simulations were used to study the conformational dynamics of these interference peptides in aqueous solution to characterise their structure and dynamics. Transitions from disordered to α-helical conformation, stabilised by hydrogen bonds and proline-aromatic interactions, were observed throughout the simulations. The backbone of the wild-type peptide folds to a similar conformation as that found in ternary complexes of anterior Hox proteins with conserved hexapeptide motifs important for recognition of pre-B-cell leukemia Homeobox 1, indicating that the motif may possess an intrinsic preference for helical structure. The predicted NMR chemical shifts of these peptides are consistent with the Hox hexapeptides in solution and Engrailed 2 NMR data. These findings highlight the importance of aromatic residues in determining the structure of Engrailed 1 interference peptides, shedding light on the rational design strategy of molecules that could be adopted to inhibit other transcription factors overexpressed in other cancer types, potentially including other transcription factor families that require highly conserved and cooperative protein–protein partnerships for biological activity.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.