Assessment of micro RNAs expression in leukemic cells as prognostic markers in chronic lymphocytic leukemia: micro RNAs can predict survival in a course of the disease
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Agnieszka Szymczyk1,2, Sylwia Chocholska1, Arkadiusz Macheta1, Dariusz Szczepanek3, Marek Hus1 and Monika Podhorecka1
1Department of Haematooncology and Bone Marrow Transplantation, Medical University of Lublin, Lublin, Poland
2Independent Clinical Transplantology Unit, Medical University of Lublin, Poland
3Department of Neurosurgery and Pediatric Neurosurgery, Medical University of Lublin, Lublin, Poland
Monika Podhorecka, email: firstname.lastname@example.org
Keywords: chronic lymphocytic leukemia; micro RNA; overall survival; prognostic factors; progression free survival
Received: February 21, 2018 Accepted: March 07, 2018 Published: April 10, 2018
Numerous genetic alterations predicting prognosis and clinical outcome are revealed recently in chronic lymphocytic leukemia (CLL). Among them the deregulated expression of micro RNAs that can induce tumor growth, or act as tumor suppressors seem to be of great importance. This study aimed to analyze the possible role of chosen micro RNAs as markers of prognosis in patients with CLL. We assessed the expression of miR-21, miR-34a, miR-181a, miR-199a/b and miR-221 in previously separated leukemic cells with the use of qRQ-PCR technique at the moment of diagnosis. The results were then analyzed in regards to presence of prognostic factors, clinical data and the end points like progression free survival (PFS), time to progression (TP) and overall survival time (OS).
We detected significant correlations between expression of the analyzed micro RNAs and CLL prognostic markers particularly as far as miR-221 and miR-181a were concerned. The subsequent analysis revealed that high expression of miR-34a and miR-181a as well as low miR-21 expression indicated longer TTP, while miR-221 was predictor of OS.
The obtained results prove the role of micro RNAs as CLL prognostic markers, particularly as factors predicting survival in a course of the disease.
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