Research Papers:

Salivary microRNAs as new molecular markers in cleft lip and palate: a new frontier in molecular medicine

Vincenzo Grassia, Angela Lombardi, Hiromichi Kawasaki, Carmela Ferri, Letizia Perillo, Laura Mosca, Donatella Delle Cave, Ludovica Nucci, Marina Porcelli and Michele Caraglia _

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Oncotarget. 2018; 9:18929-18938. https://doi.org/10.18632/oncotarget.24838

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Vincenzo Grassia1,*, Angela Lombardi2,*, Hiromichi Kawasaki2,3, Carmela Ferri2, Letizia Perillo1, Laura Mosca2, Donatella Delle Cave2, Ludovica Nucci1, Marina Porcelli2 and Michele Caraglia2

1Mutidiciplinary Department of Medical- Surgical and Dental Specialties, University of Campania “L. Vanvitelli”, Naples, Italy

2Department of Precision Medicine University of Campania “L. Vanvitelli”, Naples, Italy

3Drug Discovery Laboratory, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan

*These authors contributed equally to this work

Correspondence to:

Michele Caraglia, email: [email protected]

Keywords: miRNAs; CLP; MTHFR; SATB2; PVRL1

Received: November 26, 2017     Accepted: February 28, 2018     Published: April 10, 2018


MicroRNAs (miRNAs) are endogenous non-coding RNAs of about twenty-two nucleotides that regulate gene expression through post-transcriptional control. The purpose of the present study was to identify and describe the salivary miRNAs in cleft lip and palate (CLP) patients comparing them with a control healthy group. Twelve patients (mean age 11.9 ± 2.42 years; 6M/6F) formed the study group. The control group was created selecting twelve healthy subjects matched for age and sex with study group. We recorded differences in miRNA expression profile between the saliva of CLP patients and the control group. Specifically, miR-141, miR-223, and miR-324-3p were mostly deregulated between the study and control groups. Interestingly, these three miRNAs are the regulators of the following genes correlated to cleft palate and lip development: MTHFR, SATB2, PVRL1. The present study showed that collecting saliva samples is a non-invasive procedure and is well accepted by CLP patients. MiRNAs can be easily isolated and identified. The differences in regulation of miR-141, miR-223 and miR-324-3p between the two groups of salivary samples suggest that these molecules are valid prognostic biomarkers and therapy dynamic response indicators, also for the accuracy and non-invasive sampling and dosing system.

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