Oncotarget

Research Papers:

Differential β2-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines

Lucía Gargiulo _, Sabrina Copsel, Ezequiel M. Rivero, Céline Galés, Jean-Michel Sénard, Isabel A. Lüthy, Carlos Davio and Ariana Bruzzone

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2014; 5:10058-10069. https://doi.org/10.18632/oncotarget.2460

Metrics: PDF 1179 views  |   HTML 1733 views  |   ?  


Abstract

Lucía Gargiulo1,*, Sabrina Copsel1,2,*, Ezequiel M. Rivero1, Céline Galés3, Jean-Michel Sénard3, Isabel A. Lüthy1, Carlos Davio2,*, Ariana Bruzzone1,*

1Instituto de Biología y Medicina Experimental-CONICET, Vuelta de Obligado 2490, C1428ADN, CABA, Argentina

2Laboratorio de Farmacología de Receptores, Departamento de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956 (1113) CABA, Argentina

3Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale, U1048, Université Toulouse III Paul Sabatier, F-31432 Toulouse, France

*These authors contributed equally to this work

Correspondence Author:

Ariana Bruzzone, e-mail: ariana.bruzzone@ibyme.conicet.gov.ar

Keywords: human breast cancer cells, non-tumorigenic breast cells, epinephrine, adrenergic receptors.

Received: June 17, 2014     Accepted: September 06, 2014     Published: November 07, 2014

ABSTRACT

Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β2-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β2-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β2-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 2460