Oncotarget

Research Papers: Immunology:

DEC205 mediates local and systemic immune responses to Helicobacter pylori infection in humans

Masahide Kita _, Kenji Yokota, Chihiro Kageyama, Susumu Take, Kazuyoshi Goto, Yoshiro Kawahara, Osamu Matsushita and Hiroyuki Okada

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Oncotarget. 2018; 9:15828-15835. https://doi.org/10.18632/oncotarget.24574

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Abstract

Masahide Kita1, Kenji Yokota2, Chihiro Kageyama2, Susumu Take1, Kazuyoshi Goto3, Yoshiro Kawahara4, Osamu Matsushita3 and Hiroyuki Okada1

1Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry, and Pharmaceutical Science, Okayama University, Okayama, Japan

2Graduate School of Health Science, Okayama University, Okayama, Japan

3Department of Bacteriology, Graduate School of Medicine, Dentistry, and Pharmaceutical Science, Okayama University, Okayama, Japan

4Department of Endoscopy, Okayama University Hospital, Okayama, Japan

Correspondence to:

Kenji Yokota, email: yokochan@md.okayama-u.ac.jp

Keywords: CD14; DEC205; Helicobacter pylori; macrophage; Immunology

Received: November 04, 2016     Accepted: October 25, 2017     Epub: February 26, 2018     Published: March 23, 2018

ABSTRACT

Helicobacter pylori infections cause gastritis and affect systemic immune responses; however, no direct association between immune cells and stomach bacteria has yet been reported. The present study investigated DEC205-mediated phagocytosis of H. pylori and the role of DEC205-positive macrophages in the human gastric mucosa. DEC205 mediated phagocytosis of H. pylori was detected immunocytochemically in PMA-stimulated macrophages differentiated from NOMO1 cells. Expression of DEC205 mRNA in peripheral blood mononuclear cells (PBMCs) from H. pylori-infected patients was analyzed following stimulation with H. pylori cell lysate. We found that anti-DEC205 antibodies inhibited phagocytosis of H. pylori. The number of cells double-positive for DEC205 and CD14 in human gastric mucosa was higher in H. pylori-infected patients. DEC205-positive macrophages invaded the extracellular space between epithelial cells within gastric pits. In addition, DEC205 mRNA expression was upregulated in human PBMCs stimulated with H. pylori lysate. These findings suggest DEC205-expressing macrophages are important for recognition of H. pylori in human gastric mucosa, which affects systemic immunity.


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