Description of organ-specific phenotype, and functional characteristics of tissue resident lymphocytes from liver transplantation donor and research on immune tolerance mechanism of liver
Metrics: PDF 838 views | HTML 1984 views | ?
Yunpeng Shi1, Ping Zhang1, Guangyi Wang1, Xingkai Liu1, Xiaodong Sun1, Xin Zhang3, Haijun Li2, Jun Qi1, Lei Ding1, Ting Li1, Ruoyan Zhang1, Yuguo Chen1, Jianpeng Zhou1, Guoyue Lv1 and Zhengkun Tu1,2
1Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
2Department of Surgery, Institute of Translational Medicine, The First Hospital of Jilin University, Changchun, Jilin 130061, P.R. China
3Department of Emergency Surgery, Jilin Province People's Hospital, Changchun, Jilin 130021, P.R. China
Ping Zhang, email: firstname.lastname@example.org
Zhengkun Tu, email: email@example.com
Guoyue Lv, email: firstname.lastname@example.org
Keywords: liver transplantation; intrahepatic lymphocytes; liver immune tolerance
Received: November 28, 2017 Accepted: January 25, 2018 Epub: February 15, 2018 Published: March 20, 2018
Aim: Prior to transplantation, Donation after Cardiac Death (DCD) liver transplantation livers are perfused with preservation solution. Therefore, this provides an abundant source of human liver lymphocytes, as well as mesenteric lymph node and spleen for the study of lymphocyte subset diversity in the peripheral blood, lymph node, spleen and liver.
Methods: Lymphocyte subsets were isolated and purified from peripheral blood, lymph node, spleen and liver perfusion, the phenotypic and functional analysis of the tissue resident lymphocyte were performed by flow cytometry.
Results: In a direct comparison between blood, liver, lymph node and spleen cells from liver transplantation donors, the abundance of natural killer (NK) cells, CD3+CD56+NKT (NT) cells and CD8+ T cells in intrahapatic lymphocytes (IHL) did not match what was present in peripheral blood and other peripheral lymphoid organs. The activation state of peripheral blood-derived lymphocytes was significantly different from lymph node-, spleen- and liver-derived cells. Intriguingly, NK cells, CD4+ T cells, and CD8+ T cells from liver perfusion display more suppressive characteristics, that is, express and produce more anti-inflammatory cytokine interleukin (IL)-10, less inflammatory cytokine interferon (INF)-γ.
Conclusion: Our findings imply that different tissues entail resident lymphocyte subsets with a distinct phenotype and function considering the organ is well vascularized, particularly in liver. It is better to understand the mechanism of liver immune tolerance.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.