Interaction of renin-angiotensin system gene polymorphisms with hypertension in Chinese patients with type 1 diabetes and retinopathy
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Yong-Chao Qiao1, Yan-Hong Pan2, Yan Xu3, Xiao-Xi Zhang2,4 and Hai-Lu Zhao1,2,4
1Department of Immunology, Xiangya School of Medicine, Central South University, Changsha, Hunan, China
2Center for Diabetic Systems Medicine, Guangxi Key Laboratory of Excellence, Guilin Medical University, Guilin, Guangxi, China
3Department of Laboratory Medicine, Hunan Aerospace Hospital, Changsha, Hunan, China
4Department of Immunology, Faculty of Basic Medicine, Guilin Medical University, Guilin, Guangxi, China
Hai-Lu Zhao, email: [email protected]
Keywords: type 1 diabetes mellitus; gene polymorphisms; genotype; diabetic retinopathy; renin-angiotensin system
Received: November 19, 2017 Accepted: January 03, 2018 Published: January 09, 2018
Background: The objective of this research was to investigate the interaction of RAS gene polymorphisms in Chinese patients with type 1 diabetes mellitus (T1DM) and diabetic retinopathy (DR).
Methods: Genomic DNA was extracted from peripheral blood leukocytes and genotyping for the angiotensin converting enzyme (ACE) gene I/D and angiotensinogen (AGT) gene M/T polymorphisms was performed using the polymerase chain reaction method. 311 T1DM patients were recruited for the assessment of ACE and AGT polymorphisms relating to DR.
Results: Compared with the diabetic non-retinopathy (DNR) patients, DR patients had lower proportion of diabetic nephropathy (p<0.001) and M allele (p=0.013). Intriguingly, the frequency D allele (p=0.035) was lower in DR patients with hypertension, as well as DD (p=0.003) and DI genotype (p=0.012) in DR patients with normal blood pressure after multiple tests with Bonferroni correction, but D allele (p=0.025) displayed higher in normotensive patients with T1DM. Logistic regression analyses indicated that no significant relationship existed about the genotype and allele polymorphisms with the progress of DR after adjusting for confounding factors.
Conclusions: Interaction of hypertension and the RAS gene polymorphisms might have a role in the DR development in Chinese T1DM patients.
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