Research Papers:
Chronic feeding with proteinrestricted diets affect ileal amino acid digestibility and the expression of nutrientsensing hormone secretion gastrointestinal digestive enzyme and nutrient transporter genes in young weaned pigs
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Li Wu1,2,*, Peng Liao1,*, Qinghua He2,*, Bie Tan1, Feifei Guo3, Min Tang4 and Tiejun Li1
1Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan Province 410125, China
2Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518055, China
3Longyan Taimai and Sanluck Pharmaceutical Co., LTD., Longyan, Fujian Province 366215, China
4Department of Gynaecology and Obstetrics, Mawangdui District of Hunan Provincial People's Hospital, Changsha, Hunan Province 410016, China
*These authors contributed equally to this work
Correspondence to:
Peng Liao, email: [email protected]
Min Tang, email: [email protected]
Tiejun Li, email: [email protected]
Keywords: weaned pigs; protein restriction diets; amino acid; nutrient-sensing; digestive enzyme
Received: November 16, 2017 Accepted: January 02, 2018 Published: January 09, 2018
ABSTRACT
The aim of this work was to investigate the serum biochemical profile, ileal amino acid (AA) digestibility, and expression of nutrient-sensing, hormone secretion, gastrointestinal (GIT) digestive enzyme and nutrient transporter genes in pigs subjected to dietary protein restriction. Twenty-four weaned pigs were distributed into three treatments, and the animals in each treatment were fed crude protein (CP) diets at one of three levels (20, 17, and 14%). Our results showed that pigs fed the 20% CP diet had greater (P < 0.05) average daily gain and ratio of feed to gain than those fed the 14% CP diet, but there were no differences between the 20% CP and 17% CP diets. Additionally, the 20% CP diet tended to up-regulate (P < 0.05) the expression of nutrient-sensing-related genes, such as taste receptor type 1 member 1 (TasR1), calcium-sensing receptor (CaSR), and solute carrier family 1 (EAAC1), and the 17% CP diet tended to up-regulate (P < 0.05) the expression of digestive enzyme-related genes, hormone secretion-related genes, and nutrient transporter-related genes, such as pepsinogen, cholecystokinin type A receptor (CCK-1R), and dipeptide transporter 1 (PepT1), respectively. These results suggested that weaned pigs that were chronically fed a moderately restricted 17% CP protein diet could catch up to pigs fed a 20% CP diet in terms of growth performance and feed efficiency. In conclusion, the provision of 20% dietary CP was beneficial to the expression of nutrient-sensing and nutrient transporter genes in the GIT, and the provision of 17% dietary CP was beneficial to lipid metabolism and digestive enzymes in the GIT.