Research Papers:
Bioinformatic analysis of Listeria monocytogenes CRISPR
Metrics: PDF 860 views | Full Text 1747 views
Daofeng Qu1, Yang Shen1, Lin Xu1, Ziying Xiang1, Lu Zhang1, Yuewen Chen1, Dongping Huang1, Anna Tong1, Liying Zhang1 and Jianzhong Han1
1Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China
Correspondence to:
Jianzhong Han, email: [email protected]
Keywords: L. monocytogenes; CRISPR; repeat; Cas; plasmid
Received: July 22, 2017 Accepted: December 24, 2017 Published: January 03, 2018
ABSTRACT
Listeria monocytogenes is a leading causes of death from food-borne pathogens. Bioinformatics approach was applied to investigate the features of L. monocytogenes CRISPR structure and the relationship between CRISPR and plasmid transposase content. Among 93 L. monocytogenes genomes, 95 confirmed CRISPR structure loci were identified and classified into 5 groups based on repeat size. RNA secondary structure and minimum free energy indicated that the secondary structure of Group 5 (36 bp) was more stable than other groups. Type I-B or II-A Cas genes were found in 36 strains, and the CRISPR-Cas system of type I-B was more conserved than type II-A. Furthermore, CRISPR loci affected the enzyme transposase content of L. monocytogenes plasmid. This study examined the diversity of the CRISPR-Cas system in L. monocytogenes, classified CRISPR structure and repeats, and demonstrated the influence of the CRISPR-Cas system on the number of transposase in plasmid.