Application of a double-colour upconversion nanofluorescent probe for targeted imaging of mantle cell lymphoma
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Guang Yang1, Yong Cao1,2, Bin Yan1, Qiang Lv2, Jianbo Yu1,2, Fusheng Zhao2, Zhihong Chen2, Heran Yang1, Mengxi Chen1 and Zaishun Jin1,2
1Pathology Department, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang 157011, P.R. China
2Key Laboratory of Tumor Prevention and Treatment (Heilongjiang Higher Education Institutions), Mudanjiang Medical University, Mudanjiang 157011, P.R. China
Zaishun Jin, email: email@example.com
Keywords: upconversion fluorescence; nanoprobe; biological imaging; mantle cell lymphoma; immune labelling
Received: June 30, 2017 Accepted: October 30, 2017 Epub: December 23, 2017 Published: March 30, 2018
Upconversion nanoparticles are a new type of fluorescent marker in biomedical imaging that can convert a longer wavelength (such as near-infrared fluorescence) into a shorter wavelength (such as visible light). Mantle cell lymphoma, which is derived from B-cell lymphoma, is a subtype of non-Hodgkin's lymphoma, and the immune phenotype is a mature B-cell phenotype (CD20+, CD5+). To develop the use of nanomaterials as specific markers for the medical imaging of mantle cell lymphoma, we modified the surface of UCNPs by oxidation so that the CD20 or CD5 antibody could covalently attach to the upconversion nanoparticles to form antibody-UCNP conjugates. These antibody-UCNP conjugates were used as fluorescent probes to detect the CD20 or CD5 antigen. Due to the excessive expression of these antigens on the surface of MCL cells and successful strong connection between the antibody and UCNPs, the latter could specifically combine with mantle cell lymphoma cells. Upon near-infrared excitation at 980 nm, cells labelled with UCNPs emitted bright upconversion fluorescence.
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