The single-nucleotide polymorphisms in CHD5 affect the prognosis of patients with hepatocellular carcinoma
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Xiao Zhu1,10,11,*, Qingming Kong2,*, Liwei Xie3,*, Zhihong Chen1,*, Hongmei Li1, Zhu Zhu1, Yongmei Huang1,4, Feifei Lan5, Haiqing Luo1,6, Jingting Zhan1,4, Hongrong Ding1, Jinli Lei1,4, Qin Xiao7, Weiming Fu8, Wenguo Fan9, Jinfang Zhang12 and Hui Luo1,4
1Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, China
2Immunity and Biochemical Research Lab, Zhejiang Academy of Medical Sciences, Hangzhou, China
3Guangdong Institute of Microbiology, Guangzhou, China
4Institute of Marine Medicine Research, Guangdong Medical University, Zhanjiang, China
5Forensic Identification Institute, Guangdong Women and Children Hospital, Guangzhou, China
6The Affiliated Hospital Cancer Center, Guangdong Medical University, Zhanjiang, China
7Department of Blood Transfusion, Peking University Shenzhen Hospital, Shenzhen, China
8School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong, China
9Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China
10Cancer Center, Medical College of Georgia, Georgia Health Sciences University, Augusta, GA, USA
11Institute of Bioinformatics, University of Georgia, Athens, GA, USA
12Key Laboratory of Orthopaedics and Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China
*These authors contributed equally to this work
Hui Luo, email: firstname.lastname@example.org
Jinfang Zhang, email: email@example.com
Keywords: hepatocellular carcinoma; chromodomain-helicase-DNA-binding protein 5; prognosis; single-nucleotide polymorphisms; linkage disequilibrium
Received: May 27, 2017 Accepted: November 19, 2017 Published: December 26, 2017
Previous studies showed that the low expressions of chromodomain-helicase-DNA-binding protein 5 (CHD5) were intensively associated with deteriorative biologic and clinical characteristics as well as outcomes in many tumors. The aim of this study is to determine whether CHD5 single nucleotide polymorphisms (SNPs) contribute to the prognosis of hepatocellular carcima (HCC). The SNPs were selected according to their linkage disequilibrium (LD) in the targeted next-generation sequencing (NGS) and then genotyped with TaqMan probers. We revealed a rare haplotype AG in CHD5 (SNPs: rs12564469-rs9434711) was markedly associated with HCC prognosis. The univariate and multivariate regression analyses revealed the patients with worse overall survival time were those with tumor metastasis and haplotype AG, as well as cirrhosis, poor differentiation and IV-TNM stage. Based on the available public databases, we discovered the significant association between haplotype AG and CHD5 mRNA expressions only existed in Chinese. These data proposed that the potentially genetic haplotype might functionally contribute to HCC prognosis and CHD5 mRNA expressions.
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