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Research Papers:

A programming triangular DNA origami for doxorubicin loading and delivering to target ovarian cancer cells

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Tao Song1,2, Xun Wang1, Xin Li4 and Pan Zheng3

1College of Computer and Communication Engineering, China University of Petroleum, Qingdao 266580, China

2Departamento de Inteligencia Artificial, Universidad Politécnica de Madrid, Campus de Montegancedo, Boadilla del Monte 28660, Spain

3Faculty of Engineering, Computing and Science, Swinburne University of Technology Sarawak Campus, Kuching 93350, Malaysia

4Department of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan 430060, China

Correspondence to:

Xun Wang, email: [email protected]

Keywords: DNA nanotechnology; DNA origami; drug cargo; ovarian cancer cells

Received: September 04, 2017     Accepted: December 18, 2017     Published: December 28, 2017

ABSTRACT

DNA origamis provide plenty of platforms for constructing drug-delivery systems in nanoscale. In this work, we developed triangular DNA origami in 120nm around by M13mp18 DNA strand scaffold propagated in E. coli ER2738, which shows desired thermodynamic stability and well biocompatibility. It is tested the proposed DNA origami performance on loading doxorubicin, which is a chemotherapy medication used to treat cancer. In cell intervention experiments, the obtained complex with doxorubicin achieve acceptable performances on penetrating ovarian cancer cells and inhibiting production of A2780 ovarian cancer cells. This DNA nanotechnology provides an efficient nanoscale drug delivering systems or drug cargo system for inhibiting the reproduction of ovarian cancer cells in vitro.