Oncotarget

Research Papers:

Inhibition of autophagy-attenuated calcium oxalate crystal-induced renal tubular epithelial cell injury in vivo and in vitro

Yunlong Liu, Derong Li, Ziqi He, Quan Liu, Jihua Wu, Xiaofeng Guan, Zhiwei Tao _ and Yaoliang Deng

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Oncotarget. 2018; 9:4571-4582. https://doi.org/10.18632/oncotarget.23383

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Abstract

Yunlong Liu1, Derong Li1, Ziqi He1, Quan Liu1, Jihua Wu1, Xiaofeng Guan1, Zhiwei Tao1 and Yaoliang Deng1

1Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

Correspondence to:

Zhiwei Tao, email: 791562693@qq.com

Yaoliang Deng, email: dylkf317@163.com

Keywords: autophagy; calcium oxalate crystals; renal tubular epithelial cells; reactive oxygen species; autophagic vacuoles

Received: August 29, 2017     Accepted: November 15, 2017     Published: December 17, 2017

ABSTRACT

Accumulating evidence suggests that autophagy is involved in the pathophysiological processes of kidney diseases. However, the role of autophagy in the formation of calcium oxalate (CaOx) nephrolithiasis remains unclear. In this study, we investigated the effects of autophagy on renal tubular epithelial cell injury induced by CaOx crystals in vivo and in vitro. We first observed that the expression levels of LC3-II and BECN1 and number of autophagic vacuoles were markedly increased in the renal tissue of CaOx stone patients. We subsequently found that exposure of HK-2 cells to CaOx crystals could increase LC3-II and BECN1 expression as well as the number of GFP-LC3 dots and autophagic vacuoles in a dose- and time-dependent manner. In addition, our results suggest that CaOx crystals induced autophagy, at least in part, via activation of the reactive oxygen species (ROS) pathway in HK-2 cells. Furthermore, inhibition of autophagy using 3-methyladenine or siRNA knockdown of BECN1 attenuated CaOx crystal-induced HK-2 cells injury. However, enhancing autophagic activity with rapamycin exerted an opposite effect. Taken together, our results demonstrate that autophagy is essential for CaOx crystal-induced renal tubular epithelial cell injury and that inhibition of autophagy could be a novel therapeutic strategy for CaOx nephrolithiasis.


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