CD34− human placenta-derived mesenchymal stem cells protect against heat stroke mortality in rats
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Willie Lin1, Yogi Chang-Yo Hsuan1, Yu-Chin Su1, Cheng-Hsien Lin1, Mao-Tsun Lin2, Zi-Hao Chen3,4, Ching-Ping Chang2,4,5 and Kao-Chang Lin4,6
1Meridigen Biotech Co., Ltd., Taipei, Taiwan
2Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan
3Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
4Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan
5The Ph.D. Program for Neural Regenerative Medicine, Taipei Medical University, Taipei, Taiwan
6Department of Neurology, Chi Mei Medical Center, Tainan, Taiwan
Kao-Chang Lin, email: [email protected]
Keywords: heat stroke; placenta; mesenchymal stem cells; ischemia; inflammation
Received: August 24, 2017 Accepted: December 09, 2017 Published: December 15, 2017
CD34 is a transmembrane phosphoglycoprotein used to selectively enrich bone marrow in hematopoietic stem cells for transplantation. Treating rats with CD34+ cells derived from human umbilical cord blood before or after heat stroke has been shown to promote survival. We investigated whether CD34– human placenta-derived stem cells (PDMSCs) could improve survival following heat stroke in rats. Rats were subjected to heat stress (42°C for 98 min) to induce heat stroke. Intravenous administration of PDMSCs 1 day before or immediately after the onset of heat stroke improved survival by 60% and 20%, respectively. Pre-treatment with CD34− PDMSCs protected against heat stroke injury more effectively than that treatment after injury. PDMSCs treatment attenuated cerebrovascular dysfunction, the inflammatory response, and lipid peroxidation. These data suggest human PDMSCs protect against heat stroke injury in rats. Moreover, these effects do not require the presence of CD34+ cells.
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