Multi-color RGB marking enables clonality assessment of liver tumors in a murine xenograft model
PDF | HTML | Supplementary Files | How to cite
Metrics: PDF 1038 views | HTML 2306 views | ?
Michael Thomaschewski1, Kristoffer Riecken1, Ludmilla Unrau1, Tassilo Volz2, Kerstin Cornils1, Harald Ittrich3, Denise Heim2, Henning Wege2, Ercan Akgün1, Marc Lütgehetmann2, Jan Dieckhoff3, Michael Köpke2, Maura Dandri2, Daniel Benten2,4,* and Boris Fehse1,*
1Research Department of Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center (UMC) Hamburg-Eppendorf, Hamburg, Germany
2Department of Medicine, Gastroenterology and Hepatology, UMC Hamburg-Eppendorf, Hamburg, Germany
3Diagnostic and Interventional Radiology, UMC Hamburg-Eppendorf, Hamburg, Germany
4Department of Gastroenterology, Helios Klinikum Duisburg, Duisburg, Germany
*These authors have contributed equally to this work
Boris Fehse, email: [email protected]
Daniel Benten, email: [email protected]
Keywords: HCC; insertional mutagenesis; RGB marking; LeGO vectors; hTERT
Received: September 15, 2017 Accepted: December 04, 2017 Published: December 14, 2017
We recently introduced red-green-blue (RGB) marking for clonal cell tracking based on individual color-coding. Here, we applied RGB marking to study clonal development of liver tumors. Immortalized, non-tumorigenic human fetal hepatocytes expressing the human telomerase reverse transcriptase (FH-hTERT) were RGB-marked by simultaneous transduction with lentiviral vectors encoding mCherry, Venus, and Cerulean. Multi-color fluorescence microscopy was used to analyze growth characteristics of RGB-marked FH-hTERT in vitro and in vivo after transplantation into livers of immunodeficient mice with endogenous liver damage (uPA/SCID). After initially polyclonal engraftment we observed oligoclonal regenerative nodules derived from transplanted RGB-marked FH-hTERT. Some mice developed monochromatic invasive liver tumors; their clonal origin was confirmed both on the molecular level, based on specific lentiviral-vector insertion sites, and by serial transplantation of one tumor. Vector insertions in proximity to the proto-oncogene MCF2 and the transcription factor MITF resulted in strong upregulation of mRNA expression in the respective tumors. Notably, upregulated MCF2 and MITF expression was also observed in 21% and 33% of 24 human hepatocellular carcinomas analyzed. In conclusion, liver repopulation with RGB-marked FH-hTERT is a useful tool to study clonal progression of liver tumors caused by insertional mutagenesis in vivo and will help identifying genes involved in liver cancer.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.