Long non-coding RNA deep sequencing reveals the role of macrophage in liver disorders
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Zhang Lin1,2,3, Hao Changfu1, Zhao Fengling4, Guo Wei4, Bao Lei1, Li Yiping1, Zhang Miao1, Yue Zhongzheng1, Zhao Youliang1, Duan Shuyin1 and Yao Wu1
1Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001, China
2Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250001, China
3Key Laboratory of Reproductive Endocrinology, Shandong University, Ministry of Education, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250001, China
4Department of Occupational Disease, Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China
Yao Wu, email: [email protected]
Keywords: macrophage; liver disorder; lncRNA profiling; cancer; biomarker
Received: September 03, 2017 Accepted: November 15, 2017 Published: December 12, 2017
Liver disorders such as hepatitis, cirrhosis and hepatocellular carcinoma are a series of the most life threatening diseases along with extensive inflammatory cellular infiltrations. Macrophage has been proved to be key regulators and initiators of inflammation, and long non-coding RNAs (lncRNAs) are recommended to play critical roles in the occurrence and development of a variety of diseases. To uncover the role of macrophage in liver disorders via lncRNA sequencing method, we first applied a lncRNA classification pipeline to identify 1247 lncRNAs represented on the Affymetrix Mouse Genome 430/430A 2.0 array. We then analyzed the lncRNA expression patterns in a set of previously published gene expression profiles of silica particle exposed macrophages and liver respectively, and identified and validated sets of differentially expressed lncRNAs shared by macrophages and liver. The functional enrichment analysis of these lncRNAs was processed on the basis of their expression signatures, three aspects including cis, trans and co-acting proteins were proposed. This is the first time to correlate macrophage with liver disorders via co-expressed lncRNAs. Our findings indicated that roles of macrophage in liver disorders were double-edged, the differentially expressed lncRNAs and their corresponding regulatory genes or proteins may serve as potential diagnostic biomarkers and therapeutic targets.
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