Oncotarget

Research Papers:

MicroRNA miR-147b promotes tumor growth via targeting UBE2N in hepatocellular carcinoma

En Zhang _, Qin Liu, Yong Wang, Hui Wang, Li He, Xiuli Jin and Ning Li

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Oncotarget. 2017; 8:114072-114080. https://doi.org/10.18632/oncotarget.23120

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Abstract

En Zhang1,*, Qin Liu2,*, Yong Wang1, Hui Wang1, Li He1, Xiuli Jin1 and Ning Li3

1Department of Gastroenterology, The Second Affiliated Hospital of Shenyang Medical College, Shenyang 110035, China

2Department of Gynecology and Obstetrics, Seventh People's Hospital of Shanghai University of TCM, Shanghai 200137, China

3Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China

*These authors have contributed equally to this work

Correspondence to:

En Zhang, email: 15840552727@139.com

Ning Li, email: lining_hs@fudan.edu.cn

Keywords: hepatocellular carcinoma; tumor growth; ubiquitin-conjugating enzyme E2N (UBE2N); miR-147b; microRNA

Received: July 20, 2017     Accepted: November 30, 2017     Published: December 09, 2017

ABSTRACT

As the subfamily of noncoding RNA, microRNAs (miRNAs) broadly regulate the development of cancers, while their dysregulation and function in human hepatocellular carcinoma (HCC) remains largely unclear. Here, we found the expression level of microRNA-147b (miR-147b) is increased aberrantly in HCC tumor tissues, and its expression positively correlates to the tumor severity. In both MTT and colony formation assay, knockdown of miR-147b dramatically inhibits in vitro proliferation of HCC cell lines. More interestingly, we also performed in vivo tumorigenesis assay and found that miR-147b can regulate in vivo tumorigenesis in nude mice xenograft models. The ubiquitin-conjugating enzyme E2N (UBE2N) was identified directly and functionally targeted by miR-147b. The mRNA level of UBE2N is increased in HCC tumors or cell lines. Restoring UBE2N expression level in tumor cells leads to inhibition of cell proliferation, which mimics the effect upon miR-147b knockdown in the same cells. These data elucidated the oncogenic role of miR-147b in HCC development and progression with therapeutic target potentials.


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