Oncotarget

Research Papers: Immunology:

Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model

Yongsheng Cao, Qiya Zhang, Liming Xu, Shaowu Li, Di Wang, Jingzhuang Zhao, Hongbai Liu, Jian Feng and Tongyan Lu _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:112222-112235. https://doi.org/10.18632/oncotarget.23095

Metrics: PDF 1498 views  |   HTML 4551 views  |   ?  


Abstract

Yongsheng Cao1,2, Qiya Zhang2, Liming Xu1, Shaowu Li1, Di Wang1, Jingzhuang Zhao1, Hongbai Liu1, Jian Feng3 and Tongyan Lu1

1 Heilongjiang River Fishery Research Institute of Chinese Academy of Fishery Sciences, Harbin, China

2 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China

3 Benxi Agrimarine Company Limited, Benxi, China

Correspondence to:

Tongyan Lu, email:

Qiya Zhang, email:

Keywords: rainbow trout; cytokines; adjuvant; DNA vaccine; protection; Immune response; Immunology

Received: June 01, 2017 Accepted: November 17, 2017 Published: December 11, 2017

Abstract

Seven rainbow trout cytokine genes (interleukin (IL)-2, IL-8, IL-15, IL-17, IL-1β, intracellular interferon (iIFN) 1a, and IFN-γ2) were evaluated for their adjuvant effects on a DNA vaccine, called pG, containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV). Distinct DNA constructs in expression plasmid pcDNA3.1 encoding a cytokine gene were generated. Immunofluorescence assays in rainbow trout gonadal cells demonstrated successful protein expression from all these constructs. Subsequently, fish were immunized with pG alone or together with a cytokine expression plasmid. Results showed that each cytokine plasmids at an appropriate dose showed notable effects on immune gene expression. IL-17 and IFN-γ2 can enhance early specific IgM response. All cytokines, except IL-8, can benefit initial neutralizing antibody (NAb) titers. At 35 days post immunization (dpi), NAb titers of fish immunized with pG and IL-2, iIFN1a, or IFN-γ2 plasmids remained at high levels (1:160). NAb titers of fish immunized with pG alone decreased to 1:40. IL-8 or IL-1β can enhance antigen-specific proliferative T-cell responses at 14 dpi. At 28 dpi, coinjection of pG with IL-2, IL-8, IL-15, or IL-17 plasmids induced considerably stronger lymphocyte proliferation than that with injection of pG alone. All cytokine plasmids delivered with pG plasmid enhanced protection of trout against IHNV-mediated mortality. These results indicate that the type and dose of trout cytokine genes injected into fish affect quality of immune response to DNA vaccination.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 23095