Oncotarget

Research Papers:

Pathway-focused PCR array profiling of CAL-27 cell with overexpressed ZNF750

Li Pan, Hongli Yang, Wenqiang Tang, Cong Xu, Shuangfeng Chen, Zhen Meng, Keyi Li and Haiying Chen _

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Oncotarget. 2018; 9:566-575. https://doi.org/10.18632/oncotarget.23075

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Abstract

Li Pan1,*, Hongli Yang1,*, Wenqiang Tang1, Cong Xu1, Shuangfeng Chen1, Zhen Meng2, Keyi Li2 and Haiying Chen1

1Oral Maxillofacial Head-Neck Key Laboratory of Medical Biology, and Central Laboratory of Liaocheng People's Hospital, Liaocheng, Shandong 252000, China

2Department of Oral and Maxillofacial Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, China

*These authors contributed equally to this work

Correspondence to:

Haiying Chen, email: [email protected]

Keyi Li, email: [email protected]

Keywords: Zinc-finger protein 750 (ZNF750); oral squamous cell carcinoma (OSCC); signal transduction; PCR array

Received: August 23, 2017     Accepted: November 14, 2017     Published: December 09, 2017

ABSTRACT

Zinc-finger protein 750 (ZNF750) is the potential anti-cancer gene in oral squamous cell carcinoma (OSCC). The present study was to investigate the expression changes of ZNF750 in OSCC tissue and to reveal the induction of altered mRNA expression profiles caused by over-expressed ZNF750 in CAL-27 cell. The expression level of ZNF750 in tissue specimens from OSCC patients was detected by immunohistochemistry. Gene expression profiling was performed using Human Signal Transduction PathwayFinder RT² Profiler™ PCR Array. The expression changes of 84 key genes representing 10 signal transduction pathways in human following over-expressed ZNF750 in CAL-27 cell was examined. The expression of ZNF750 protein was reduced in OSCC tissues. The R2 PCR Array analysis revealed that 39 of the 84 examined genes that changed at least a two-fold between control and ZNF750 groups. These genes related to oxidative stress, WNT, JAK/STAT, TGFβ, NF-kappaB (NFκB), p53, Notch, Hedgehog, PPAR and Hypoxia signaling. ZNF750 could inhibit the candidate genes ATF4, SQSTM1, HMOX1, CCND1, TNF-alpha, TNFSF10 and FOSL1 but activate CDKN1A and EMP1. Our studies suggest that ZNF750 can regulate signaling pathways that related to proliferation, cell cycle, inflammation and oxidative stress in CAL-27 cell.


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