Helicobacter pylori CagA protein activates Akt and attenuates chemotherapeutics-induced apoptosis in gastric cancer cells
Metrics: PDF 972 views | HTML 1380 views | ?
Keng-Hsueh Lan1, Wei-Ping Lee2,7, Yu-Shan Wang3, Shi-Xian Liao4 and Keng-Hsin Lan4,5,6
1Division of Radiation Oncology, Department of Oncology, National Taiwan University Hospital National Taiwan University Cancer Center, Taipei, Taiwan
2Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan
3Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan
4Division of Gastroenterology and Hepatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan
5Department of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan
6Department and Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan
7Department and Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan
Keng-Hsin Lan, email: email@example.com
Keywords: Helicobacter pylori; CagA; Akt; chemotherapeutics
Received: July 21, 2017 Accepted: November 13, 2017 Published: December 09, 2017
Infection with cagA-positive Helicobacter pylori is associated with a higher risk of gastric cancer. The cagA gene product, CagA, is translocated into gastric epithelial cells and perturbs host cellular biological functions. Etoposide, a topoisomerase II inhibitor widely used to couple DNA damage to apoptosis, is a common cytotoxic agent used for advanced gastric cancer. We investigate the effect of CagA on etoposide-induced apoptosis in gastric cancer cells to elucidate whether CagA play a role in gastric carcinogenesis via impairing DNA damage-dependent apoptosis. AGS cell lines stably expressing CagA isolated from H. pylori 26695 strain were established. In the presence of etoposide, viability of parental AGS cells was decreased in a time-and dose-dependent manner, whereas CagA-expressing AGS cells were less susceptible to etoposide induced cell-killing effect. Suppression of etoposide-induced apoptosis was shown in CagA-expressing but not in parental AGS cells by DNA fragmentation, cell cycle, and annexin-V assays. This inhibitory effect of etoposide-induced apoptosis conferred by CagA was also demonstrated in SCM1 and MKN45 gastric cancer cell lines, with two additional chemotherapeutics, 5-FU and cisplatin. The effect of Akt activation on inhibition of etoposide-induced cytotoxicity by CagA was also evaluated. CagA expression and etoposide administration activate Akt in a dose-dependent manner. Enhancement of etoposide cytotoxicity by a PI-3-kinase inhibitor, LY294002, was evident in parental but was attenuated in CagA-expressing AGS cells. CagA may activate Akt, either in the absence or presence of etoposide, potentially contributing to gastric carcinogenesis associated with H. pylori infection and therapeutic resistance by impairing DNA damage-dependent apoptosis.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.