Research Papers:

Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites

Shan Xu, XiaoBo Du, Gang Feng, Yu Zhang, Jie Li, Binwei Lin, LingLin Yang, ShaoZhi Fu and JingBo Wu _

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2018; 9:282-292. https://doi.org/10.18632/oncotarget.22965

Metrics: PDF 1552 views  |   HTML 2443 views  |   ?  


Shan Xu1, Xiaobo Du1, Gang Feng1, Yu Zhang1, Jie Li1, Binwei Lin1, Linglin Yang2, Shaozhi Fu2 and Jingbo Wu2

1Department of Oncology, Mianyang Central Hospital, Mianyang 621000, Sichuan Province, China

2Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China

Correspondence to:

Jingbo Wu, email: [email protected]

Shaozhi Fu, email: [email protected]

Shan Xu, email: [email protected]

Keywords: cervical cancer; cisplatin; paclitaxel; thermosensitive hydrogel; micelles

Received: August 24, 2017     Accepted: November 09, 2017     Published: December 06, 2017


Background and Purpose: We aimed to explore the anti-tumor effect and mechanism of the combination of cisplatin (DDP)-containing thermosensitive hydrogel (PEG-PCL-PEG, or PECE) and paclitaxel (PTX)-loaded MPEG-PCL polymeric micelles called PDMP on human cervical carcinoma (HeLa) cell. In our previous studies, we found that PDMP in situ treatment of lung cancer will be liable to have potential in Lung cancer patients.

Results: Compared with other treatments, PDMP was most effective in prolonging survival time (P < 0.05), inhibiting tumor growth (P < 0.05), decreasing expression of CD133 (P < 0.05), CD31 (P < 0.05), and aldehyde dehydrogenase 1 (ALDH1) (P > 0.05), inducing G1 phase arrest (P < 0.05), increasing the apoptosis rate (P < 0.05), and in expressing ATM and γ-H2AX (P < 0.05).

Conclusions: PDMP is regarded as a promising anti-tumor reactant, when it comes to the treatment of cervical carcinoma.

Methods: we used a xenograft cervical cancer model to verify the anti-tumor activity of PDMP and to explore its mechanism of action. Mice were intratumorally administered with NS, PECE, PTX+DDP or PDMP. After two days of treatment, three mice per group were sacrificed and tumor tissue was harvested. Levels of histone H2AX phosphorylation (γ-H2AX) were determined by immunohistochemistry and ataxia telangiectasia mutated(ATM) protein levels were measured by western blot analysis. In addition, it would sacrifice each of group of three mice through 10 days’ treatment, what’s more, it would harvest tumor by virtue of flow cytometry and immunohistochemical analysis. It would like to use there maining mice to analyze tumor growth and survival. The remaining mice were analyzed for tumor growth and survival.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 22965