Research Papers:

Chitosan composite scaffold combined with bone marrow-derived mesenchymal stem cells for bone regeneration: in vitro and in vivo evaluation

Shengqi Zang, Lei Zhu, Kefu Luo, Rui Mu, Feng Chen, Xiaocui Wei, Xiaodong Yan, Biyao Han, Xiaolei Shi, Qintao Wang and Lei Jin _

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Oncotarget. 2017; 8:110890-110903. https://doi.org/10.18632/oncotarget.22917

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Shengqi Zang1,*, Lei Zhu2,*, Kefu Luo3, Rui Mu1,4, Feng Chen1, Xiaocui Wei1,4, Xiaodong Yan1, Biyao Han4, Xiaolei Shi1, Qintao Wang5 and Lei Jin1,4

1Department of Stomatology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China

2Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi 710023, P.R. China

3Department of Stomatology, Urumqi General Hospital of PLA, Urumqi 830000, P.R. China

4Medical School of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China

5Department of Periodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi 710023, P.R. China

*These authors contributed equally to this work

Correspondence to:

Lei Jin, email: [email protected]

Qintao Wang, email: [email protected]

Keywords: chitosan; bovine-derived xenograft; bioscaffold; tissue engineering; calvarial defect

Received: August 16, 2017     Accepted: October 30, 2017     Published: December 05, 2017


The study aimed to develop a chitosan (CS)-based scaffold for repairing calvarial bone defects. We fabricated composite scaffolds made of CS and bovine-derived xenograft (BDX), characterized their physicochemical properties including pore size and porosity, absorption, degradation, and compressive strength, compared their efficacy to support in vitro proliferation and differentiation of human jaw bone marrow-derived mesenchymal stem cells (hJBMMSCs), and evaluated their bone regeneration capacity in critical-size rat calvarial defects. The CS/BDX (mass ratio of 40:60) composite scaffold with porosity of 46.23% and pore size of 98.23 μm exhibited significantly enhanced compressive strength than the CS scaffold (59.33 ± 4.29 vs. 18.82 ± 2.49 Kpa). The CS/BDX (40:60) scaffold induced better cell attachment and promoted more osteogenic differentiation of hJBMMSCs than the CS scaffold. The CS/BDX (40:60) scaffold seeded with hJBMMSCs was the most effective in supporting new bone formation, as evidenced by better histomorphometry results, larger new bone area, and more obvious mature lamellar bone formation compared to other groups in rat calvarial defects 8 weeks after implantation. These results suggest that CS/BDX composite scaffold combining with hJBMMSCs has the potential for bone defect regeneration.

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