Chitosan composite scaffold combined with bone marrow-derived mesenchymal stem cells for bone regeneration: in vitro and in vivo evaluation
Metrics: PDF 776 views | HTML 1336 views | ?
Shengqi Zang1,*, Lei Zhu2,*, Kefu Luo3, Rui Mu1,4, Feng Chen1, Xiaocui Wei1,4, Xiaodong Yan1, Biyao Han4, Xiaolei Shi1, Qintao Wang5 and Lei Jin1,4
1Department of Stomatology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China
2Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi 710023, P.R. China
3Department of Stomatology, Urumqi General Hospital of PLA, Urumqi 830000, P.R. China
4Medical School of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
5Department of Periodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi 710023, P.R. China
*These authors contributed equally to this work
Lei Jin, email: firstname.lastname@example.org
Qintao Wang, email: email@example.com
Keywords: chitosan; bovine-derived xenograft; bioscaffold; tissue engineering; calvarial defect
Received: August 16, 2017 Accepted: October 30, 2017 Published: December 05, 2017
The study aimed to develop a chitosan (CS)-based scaffold for repairing calvarial bone defects. We fabricated composite scaffolds made of CS and bovine-derived xenograft (BDX), characterized their physicochemical properties including pore size and porosity, absorption, degradation, and compressive strength, compared their efficacy to support in vitro proliferation and differentiation of human jaw bone marrow-derived mesenchymal stem cells (hJBMMSCs), and evaluated their bone regeneration capacity in critical-size rat calvarial defects. The CS/BDX (mass ratio of 40:60) composite scaffold with porosity of 46.23% and pore size of 98.23 μm exhibited significantly enhanced compressive strength than the CS scaffold (59.33 ± 4.29 vs. 18.82 ± 2.49 Kpa). The CS/BDX (40:60) scaffold induced better cell attachment and promoted more osteogenic differentiation of hJBMMSCs than the CS scaffold. The CS/BDX (40:60) scaffold seeded with hJBMMSCs was the most effective in supporting new bone formation, as evidenced by better histomorphometry results, larger new bone area, and more obvious mature lamellar bone formation compared to other groups in rat calvarial defects 8 weeks after implantation. These results suggest that CS/BDX composite scaffold combining with hJBMMSCs has the potential for bone defect regeneration.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.