Oncotarget

Research Papers:

Differential gene expression profiles between two subtypes of ischemic stroke with blood stasis syndromes

Tian-Long Liu, Min-Na Liu, Xin-Liang Xu, Wen-Xing Liu, Pei-Jin Shang, Xiao-Hu Zhai, Hang Xu, Yi Ding _, Yu-Wen Li and Ai-Dong Wen

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Oncotarget. 2017; 8:111608-111622. https://doi.org/10.18632/oncotarget.22877

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Abstract

Tian-Long Liu1,2,*, Min-Na Liu3,4,*, Xin-Liang Xu5,6,*, Wen-Xing Liu1,*, Pei-Jin Shang1, Xiao-Hu Zhai1, Hang Xu1, Yi Ding1, Yu-Wen Li1,7 and Ai-Dong Wen1

1Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an, China

2Department of Pharmacy, 25th Hospital of PLA, Jiuquan, China

3Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi’an, China

4State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi’an, China

5School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, China

6Department of Traumatic Surgery, Jining No.1 Peoples Hospital, Jining, China

7Department of Pharmacy, The First Affiliated Hospital of SooChow University, Suzhou, China

*These authors have contributed equally to this work

Correspondence to:

Ai-Dong Wen, email: adwen-2004@hotmail.com

Yu-Wen Li, email: liyuwenzs@gmail.com

Yi Ding, email: dingyi.007@163.com

Keywords: ischemic stroke; blood stasis syndrome; traditional Chinese medicine; transcriptomics; network analysis

Received: August 16, 2017     Accepted: November 17, 2017     Published: December 04, 2017

ABSTRACT

Ischemic stroke is a cerebrovascular thrombotic disease with high morbidity and mortality. Qi deficiency blood stasis (QDBS) and Yin deficiency blood stasis (YDBS) are the two major subtypes of ischemic stroke according to the theories of traditional Chinese medicine. This study was conducted to distinguish these two syndromes at transcriptomics level and explore the underlying mechanisms. Male rats were randomly divided into three groups: sham group, QDBS/MCAO group and YDBS/MCAO group. Morphological changes were assessed after 24 h of reperfusion. Microarray analysis with circulating mRNA was then performed to identify differential gene expression profile, gene ontology and pathway enrichment analyses were carried out to predict the gene function, gene co-expression and pathway networks were constructed to identify the hub biomarkers, which were further validated by western blotting and Tunel staining analysis. Three subsets of dysregulated genes were acquired, including 445 QDBS-specific genes, 490 YDBS-specific genes and 1676 blood stasis common genes. Our work reveals for the first time that T cell receptor, MAPK and apoptosis pathway were identified as the hub pathways based on the pathway networks, while Nfκb1, Egfr and Casp3 were recognized as the hub genes by co-expression networks. This research helps contribute to a clearer understanding of the pathological characteristics of ischemic stroke with QDBS and YDBS syndrome, the proposed biomarkers might provide insight into the accurate diagnose and proper treatment for ischemic stroke with blood stasis syndrome.


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