Association of p53 and mitochondrial gene with chemosensitization by metformin in ovarian cancer
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De-Hua Wei1, Yan Wang1 and Hui-Rong Shi2
1Department of Gynecology, Puyang People's Hospital Affiliated to Xinxiang Medical University, Henan 457000, China
2Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
De-Hua Wei, email: [email protected]
Keywords: ovarian cancer; metformin; p53; D-loop region; chemosensitivity
Received: July 05, 2017 Accepted: November 03, 2017 Published: December 02, 2017
Objective: This study aims to investigate the association of p53 and D-loop gene with drug resistance and sensitization induced by metformin in ovarian cancer.
Results: Metformin suppresses cells in a time-dependent manner, but the inhibition does not change with the dose of metformin. This suggests that within a certain range of concentration in the body, metformin has a constant inhibitory effect on cells; and the long-term use of metformin yields a better effect.
Conclusions: Metformin enhances the sensitivity of drug-resistant ovarian cancer cells to chemotherapy.
Methods: The third passage cells of 17 cancerous ovarian tissues, which were successfully passaged five times, were used as the study objects; and the SKOV3 cell line was used as the positive control. After three adaptations, cells were cultured for 72 hours in an orthogonal experiment using drugs that were used for adaptation. Then, the inhibitory rate on cells in the experimental group was observed by CCK8 assay, in order to study the sensitization effect of metformin in different chemotherapies of ovarian cancer.
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