Research Papers:

Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK

HX Liu, MQ Xu, SP Li, S Tian, MX Guo, JY Qi, CJ He and XS Zhao _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:110566-110575. https://doi.org/10.18632/oncotarget.22821

Metrics: PDF 1188 views  |   HTML 2051 views  |   ?  


HX Liu1, MQ Xu1, SP Li1, S Tian1, MX Guo1, JY Qi1, CJ He1 and XS Zhao1

1Jujube Scientific Research and Applied Center, Life Science College, Luoyang Normal University, Luoyang, China

Correspondence to:

Zhao XS, email: [email protected]; [email protected]

Keywords: jujube leaf green tea extracts; hepatocellular carcinoma; AMPK signaling

Received: November 01, 2017     Accepted: November 13, 2017     Published: November 30, 2017


Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the normal hepatocytes. JLGTE activated AMP-activated protein kinase (AMPK) signaling, which was required for its cytotoxicity against hepatocellular carcinoma cells. Silence of AMPKα1, via targeted short hairpin RNAs or CRISPR-Cas9 genome editing, inhibited JLGTE-induced AMPK activation and HepG2 cell apoptosis. Further, in-activation of AMPK by a dominant negative AMPKα1 (T172A) also alleviated JLGTE’s cytotoxicity against HepG2 cells. On the other hand, forced-activation of AMPK by introduction of a constitutively-active AMPKα1 (T172D) mimicked JLGTE’s actions and led to HepG2 cell apoptosis. These results suggest that JLGTE inhibits human hepatocellular carcinoma cells possibly via activating AMPK.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 22821