Oncotarget

Research Papers:

N-AC-l-Leu-PEI-mediated miR-34a delivery improves osteogenic differentiation under orthodontic force

Wenwen Yu, Yi Zheng, Zhujun Yang, Hongbo Fei, Yang Wang, Xu Hou, Xinhua Sun _ and Yuqin Shen

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Oncotarget. 2017; 8:110460-110473. https://doi.org/10.18632/oncotarget.22790

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Abstract

Wenwen Yu1, Yi Zheng2, Zhujun Yang1, Hongbo Fei2, Yang Wang1, Xu Hou1, Xinhua Sun1 and Yuqin Shen2

1Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China

2Department of Periodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China

Correspondence to:

Xinhua Sun, email: xinhuasun8@163.com

Yuqin Shen, email: shen_yuqin@163.com

Keywords: miR-34a; osteogenic differentiation; orthodontic tooth movement; strain; N-acetyl-L-leucine-polyethylenimine

Received: July 31, 2017     Accepted: November 14, 2017     Published: November 30, 2017

ABSTRACT

Rare therapeutic genes or agents are reported to control orthodontic bone remodeling. MicroRNAs have recently been associated with bone metabolism. Here, we report the in vitro and in vivo effects of miR-34a on osteogenic differentiation under orthodontic force using an N-acetyl-L-leucine-modified polyethylenimine (N-Ac-l-Leu-PEI) carrier. N-Ac-l-Leu-PEI exhibited low cytotoxicity and high miR-34a transfection efficiency in rat bone mineral stem cells and local alveolar bone tissue. After transfection, miR-34a enhanced the osteogenic differentiation of Runx2 and ColI, Runx2 and ColI protein levels, and early osteogenesis function under orthodontic strain in vitro. MiR-34a also enhanced alveolar bone remodeling under orthodontic force in vivo, as evidenced by elevated gene and protein expression, upregulated indices of alveolar bone anabolism, and diminished tooth movement. We determined that the mechanism miR-34a in osteogenesis under orthodontic force may be associated with GSK-3β. These results suggested that miR-34a delivered by N-Ac-l-Leu-PEI could be a potential therapeutic target for orthodontic treatment.


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