N-AC-l-Leu-PEI-mediated miR-34a delivery improves osteogenic differentiation under orthodontic force
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Wenwen Yu1, Yi Zheng2, Zhujun Yang1, Hongbo Fei2, Yang Wang1, Xu Hou1, Xinhua Sun1 and Yuqin Shen2
1Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China
2Department of Periodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China
Xinhua Sun, email: firstname.lastname@example.org
Yuqin Shen, email: email@example.com
Keywords: miR-34a; osteogenic differentiation; orthodontic tooth movement; strain; N-acetyl-L-leucine-polyethylenimine
Received: July 31, 2017 Accepted: November 14, 2017 Published: November 30, 2017
Rare therapeutic genes or agents are reported to control orthodontic bone remodeling. MicroRNAs have recently been associated with bone metabolism. Here, we report the in vitro and in vivo effects of miR-34a on osteogenic differentiation under orthodontic force using an N-acetyl-L-leucine-modified polyethylenimine (N-Ac-l-Leu-PEI) carrier. N-Ac-l-Leu-PEI exhibited low cytotoxicity and high miR-34a transfection efficiency in rat bone mineral stem cells and local alveolar bone tissue. After transfection, miR-34a enhanced the osteogenic differentiation of Runx2 and ColI, Runx2 and ColI protein levels, and early osteogenesis function under orthodontic strain in vitro. MiR-34a also enhanced alveolar bone remodeling under orthodontic force in vivo, as evidenced by elevated gene and protein expression, upregulated indices of alveolar bone anabolism, and diminished tooth movement. We determined that the mechanism miR-34a in osteogenesis under orthodontic force may be associated with GSK-3β. These results suggested that miR-34a delivered by N-Ac-l-Leu-PEI could be a potential therapeutic target for orthodontic treatment.
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