Oncotarget

Research Papers:

Regulation of phosphate transport and AMPK signal pathway by lower dietary phosphorus of broilers

Zhiqiang Miao, Yan Feng, Junzhen Zhang, Wenxia Tian, Jianhui Li and Yu Yang _

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Oncotarget. 2017; 8:107825-107832. https://doi.org/10.18632/oncotarget.22609

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Abstract

Zhiqiang Miao1, Yan Feng1, Junzhen Zhang1, Wenxia Tian1, Jianhui Li1 and Yu Yang1

1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China

Correspondence to:

Yu Yang, email: sxauywd@126.com

Jianhui Li, email: 504387687@qq.com

Keywords: AMPK signal pathway; broilers; lower dietary phosphorus; phosphate transport regulation

Received: September 30, 2017     Accepted: October 29, 2017     Published: November 22, 2017

ABSTRACT

Lower available P (aP) was used as a base value in nutritional strategies for mitigating P pollution by animal excreta. We hypothesized that the mechanism regulating phosphate transport under low dietary P might be related with the AMPK signal pathway. A total of 144 one-day-old Arbor Acres Plus broilers were randomly allocated to control (HP) or trial (LP) diets, containing 0.45 and 0.23% aP, respectively. Growth performance, blood, intestinal, and renal samples were tested in 21-day-old broilers. Results shown that LP decreased body weight gain and feed intake. Higher serum Ca and fructose, but lower serum P and insulin were detected in LP-fed broilers. NaPi-IIb mRNA expression in intestine and NaPi-IIa mRNA expression in kidney were higher in the LP group. AMP: ATP, p-AMPK: total AMPK, and p-ACC: total ACC ratios in the duodenal mucosa were decreased in the LP group, whereas the p-mTOR: total mTOR ratio increased. These findings suggested that the increase in phosphate transport owing to LP diet might be regulated either directly by higher mTOR activity or indirectly by the suppressive AMPK signal, with corresponding changes in blood insulin and fructose content. A novel viewpoint on the regulatory mechanism underlying phosphate transport under low dietary P conditions was revealed, which might provide theoretical guidelines for reducing P pollution by means of nutritional regulation.


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