Oncotarget

Research Papers:

A premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups B, D, and E

WeiGuo Chen, Yang Liu, Aijun Li, Xinjian Li, Hongxing Li, Zhenkai Dai, Yiming Yan, Xinheng Zhang, Dingming Shu, Huanmin Zhang, Wencheng Lin, Jingyun Ma and Qingmei Xie _

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Oncotarget. 2017; 8:105942-105956. https://doi.org/10.18632/oncotarget.22512

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Abstract

WeiGuo Chen1,5,6,*, Yang Liu1,5,*, Aijun Li2, Xinjian Li1,5, Hongxing Li1,5, Zhenkai Dai1,5, Yiming Yan1,5, Xinheng Zhang1,5, Dingming Shu3, Huanmin Zhang4, Wencheng Lin1,5,6, Jingyun Ma1,5,6 and Qingmei Xie1,5,6

1College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, P. R. China

2College of Science and Engineering, Jinan University, Guangzhou 510632, P. R. China

3Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, P. R. China

4USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA

5Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China

6South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China

*These authors have contributed equally to this work

Correspondence to:

Qingmei Xie, email: qmx@scau.edu.cn

Keywords: avian leukosis virus; tvb receptor gene; premature stop codon; host resistance; chicken

Received: June 15, 2017    Accepted: November 03, 2017    Published: November 18, 2017

ABSTRACT

Avian leukosis virus (ALV) is an oncogenic virus causing a variety of neoplasms in chickens. The group of avian leukosis virus in chickens contains six closely related subgroups, A to E and J. The prevalence of ALVs in hosts may have imposed strong selective pressure toward resistance to ALVs infection. The tvb gene encodes Tvb receptor and determines susceptibility or resistance to the subgroups B, D, and E ALV. In this study, we characterized a novel resistant allele of the tvb receptor gene, tvbr3, which carries a single-nucleotide substitution (c.298C>T) that constitutes a premature termination codon within the fourth exon and leads to the production of a truncated TvbR3 receptor protein. As a result, we observed decreased susceptibility to infection by ALV-B, ALV-D and ALV-E both in vitro and in vivo, and decreased the binding affinity of the TvbR3 receptor for the subgroups B, D, and E ALV envelope glycoproteins. Additionally, we found that the tvbr3 allele was prevalent in Chinese broiler lines. This study demonstrated that premature termination codon in the tvb receptor gene can confer genetic resistance to subgroups B, D, and E ALV in the host, and indicates that tvbr3 could potentially serve as a resistant target against ALV-B, ALV-D and ALV-E infection.


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