Oncotarget

Research Papers:

Calsyntenin-1, clusterin and neutrophil gelatinase-associated lipocalin are candidate serological biomarkers for lung adenocarcinoma

Yen Chu, Yu-Heng Lai, Ming-Cheng Lee, Yu-Jung Yeh, Yao-Kuang Wu, Wayne Tsao, Chun-Yao Huang _ and Semon Wu

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Oncotarget. 2017; 8:107964-107976. https://doi.org/10.18632/oncotarget.22438

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Abstract

Yen Chu1, Yu-Heng Lai2, Ming-Cheng Lee3, Yu-Jung Yeh4, Yao-Kuang Wu5, Wayne Tsao3, Chun-Yao Huang5 and Semon Wu3,6

1Department of Medical Research and Development, Chang Gung Memorial Hospital, Graduate Institute of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan

2Department of Chemistry, Chinese Culture University, Taipei, Taiwan

3Department of Research, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, Taipei, Taiwan

4Department of Mathematics & Statistics, San Jose State University, San Jose, California, USA

5Department of Pulmonary and Critical Care, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, Taipei, Taiwan

6Department of Life Science, Chinese Culture University, Taipei, Taiwan

Correspondence to:

Chun-Yao Huang, email: huang6693@yahoo.com.tw

Semon Wu, email: semonwu@yahoo.com.tw

Keywords: serological biomarkers; non-small cell lung cancer; proteomic

Received: December 16, 2016     Accepted: October 28, 2017     Published: November 14, 2017

ABSTRACT

It has been drawn attention that secreted proteins with signal peptide from cancer cells provide new potential biomarkers of cancer. In this study, three lung adenocarcinoma cell lines and serum samples from 20 patients were used for identifying potential serologic tumor biomarker with proteomic and bioinformatics approaches. One-dimensional electrophoresis, and identified with mass spectrometry and database research were performed. We found17 secreted proteins in common, while another 17 proteins with signal peptide were identified in all three lung adenocarcinoma cell lines alone with patient samples. With matching these two groups of identified proteins, calsyntenin-1 (CLSTN1), clusterin (CLU) and neutrophil gelatinase-associated lipocalin (NGAL) were found highly secreted from both cell lines and serum with unique signal peptides. Therefore, in our study, we demonstrated that cancer cells secret specific proteins to the environment that may serve as unique markers for cancer diagnosis. To combination of proteomic study with bioinformatic prediction on signal peptides, higher expression level of CLSTN1, CLU and NGAL were found and may be new solid serologic biomarkers for patients with lung adenocarcinoma.


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