Research Papers:

Suppression of microRNA-9-5p rescues learning and memory in chronic cerebral hypoperfusion rats model

Na Wei, Kai Zheng, Rui Xue, Sheng-Li Ma, Hua-Yan Ren, Hui-Fen Huang, Wei-Wei Wang, Jing-Jing Xu _ and Kui-Sheng Chen

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Oncotarget. 2017; 8:107920-107931. https://doi.org/10.18632/oncotarget.22415

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Na Wei1,2,3,*, Kai Zheng4,*, Rui Xue5, Sheng-Li Ma6, Hua-Yan Ren1,2,3, Hui-Fen Huang1,2,3, Wei-Wei Wang1,2,3, Jing-Jing Xu1,2,3 and Kui-Sheng Chen1,2,3

1Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, People’s Republic of China

2Henan Key Laboratory of Tumor Pathology, Zhengzhou 450002, People’s Republic of China

3Department of Pathology, School of Basic Medicine, Zhengzhou University, Zhengzhou 450002, People’s Republic of China

4Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

5Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, People’s Republic of China

6Department of Emergency, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, People’s Republic of China

*These authors have contributed equally to this work

Correspondence to:

Jing-Jing Xu, email: xujingjing_1982@163.com

Kui-Sheng Chen, email: kuishengchen@zzu.edu.cn

Keywords: miR-9-5p; memory; chronic cerebral hypoperfusion; long-term potentiation

Received: May 12, 2017    Accepted: August 17, 2017    Published: November 11, 2017


Chronic cerebral hypoperfusion has been associated with cognitive impairment in dementias, such as Alzheimer’s disease (AD) and vascular disease (VaD), the two most common neurodegenerative diseases in aged people. However, the effective therapeutic approaches for both AD and VaD are still missing. MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in the epigenetic regulation in many neurological disorders; the critical roles of miRNAderegulation had been implicated in both AD and VaD. In the current study, we reported that miR-9-5p is elevated in the serum and cerebrospinalfluid of patientswith VaD. The miR-9-5p wasalso increased in both the hippocampus and cortex of rats with 2-vessel occlusionsurgery. Furthermore, application ofmiR-9-5p antagomirs attenuated the memory impairments in rats with 2-vessel occlusion surgery both in the Morris water maze and inhibitory avoidance step-down tasks. Furthermore, miR-9-5p antagomirs reducedthe inhibition oflong-term potentiation and loss of dendritic spines in chronic cerebral hypoperfusionrats. Additionally, the cholinergic neuronal function was rescued by miR-9-5p antagomirs, as well as the neuronal loss and the oxidative stress. We concluded that miR-9-5p inhibition may be a potential therapeutic target for the memory impairments caused by chronic cerebral hypoperfusion.

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