MiR-20a-5p promotes radio-resistance by targeting NPAS2 in nasopharyngeal cancer cells
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Fangfang Zhao1,*, Youguang Pu1,*, Liting Qian2, Chunbao Zang3, Zhenchao Tao3 and Jin Gao2
1The Institute of Cancer Research, Anhui Cancer Hospital, West Branch of Anhui Provincial Hospital, Anhui Medical University, Hefei 230031, Anhui, China
2Department of Radiation Oncology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230031, Anhui, China
3Department of Radiation Oncology, Anhui Cancer Hospital, West Branch of Anhui Provincial Hospital, Anhui Medical University, Hefei 230031, Anhui, China
*These authors have contributed equally to this work
Jin Gao, email: [email protected]
Keywords: nasopharyngeal cancer; miR-20a-5p; NPAS2; radio-resistance
Received: August 24, 2016 Accepted: September 13, 2017 Published: November 11, 2017
MicroRNAs (miRNAs) are key players of gene expression involved in diverse biological processes including the cancer radio-resistance, which hinders the effective cancer therapy. Here we found that the miR-20a-5p level is significantly up-regulated in radio-resistant nasopharyngeal cancer (NPC) cells via an RNA-seq and miR-omic analysis. Moreover, we identified that the neuronal PAS domain protein 2 (NPAS2) gene is one of the targets of miR-20a-5p. The involvement of miR-20a-5p and NPAS2 with NPC radio-resistance was further validated by either down- or up-regulation of their levels in NPC cell lines. Taken together, these results not only reveal novel insights into the NPC radio-resistance, but also provide hints for an effective therapeutic strategy to fight against NPC radio-resistance.
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