Oncotarget

Research Papers:

Two new species of betatorqueviruses identified in a human melanoma that metastasized to the brain

Terry Fei Fan Ng _, Jennifer A. Dill, Alvin C. Camus, Eric Delwart and Erwin G. Van Meir

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Oncotarget. 2017; 8:105800-105808. https://doi.org/10.18632/oncotarget.22400

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Abstract

Terry Fei Fan Ng1,2,3,5, Jennifer A. Dill3, Alvin C. Camus3, Eric Delwart1,2,* and Erwin G. Van Meir4,*

1Blood Systems Research Institute, San Francisco, California, USA

2Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California, USA

3Department of Pathology, University of Georgia, Athens, Georgia, USA

4Departments of Neurosurgery and Hematology & Medical Oncology, Winship Cancer Institute and School of Medicine, Emory University, Atlanta, Georgia, USA

5Current/Present address: DVD, NCIRD, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

*Co-senior authors

Correspondence to:

Terry Fei Fan Ng, email: ylz9@cdc.gov

Keywords: brain tumor; neuro-oncology; anellovirus; metagenomics; metastasis

Received: September 19, 2017     Accepted: October 25, 2017     Published: November 11, 2017

ABSTRACT

The role of viral infections in the etiology of brain cancer remains uncertain. Prior studies mostly focused on transcriptome or viral DNA integrated in tumor cells. To investigate for the presence of viral particles, we performed metagenomics sequencing on viral capsid-protected nucleic acids from 12 primary and 8 metastatic human brain tumors. One brain tumor metastasized from a skin melanoma harbored two new human anellovirus species, Torque teno mini virus Emory1 (TTMV Emory1) and Emory2 (TTMV Emory2), while the remaining 19 samples did not reveal any exogenous viral sequences. Their genomes share 63-67% identity with other TTMVs, and phylogenetic clustering supports their classification within the Betatorquevirus genus. This is the first identification of betatorqueviruses in brain tumors. The viral DNA was in its expected non-integrated circular form, and it is unclear if the viruses contributed to tumor formation. Whether the viruses originated from blood, or the primary skin tumor could not be ascertained. Overall, our results demonstrate the usefulness of viral metagenomics to detect previously unknown exogenous virus in human brain tumors. They further suggest that active viral infections are rare events in brain tumors, but support a follow-up larger scale study to quantify their frequency in different brain tumor subtypes.


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