Lipopolysaccharide-coated CuS nanoparticles promoted anti-cancer and anti-metastatic effect by immuno-photothermal therapy
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Bian Jang1,2,3,4,*, Li Xu1,*, Madhappan S. Moorthy2, Wei Zhang1, Ling Zeng1, Mingyeong Kang5, Minseok Kwak2,5, Junghwan Oh2,3,4 and Jun-O Jin1
1Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai, China
2Marine-Integrated Bionics Research Center, Pukyong National University, Busan, South Korea
3Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, South Korea
4Interdisciplinary Program of Biomedical Mechanical & Electrical Engineering, Pukyong National University, Busan, South Korea
5Department of Chemistry, Pukyong National University, Busan, South Korea
*These authors have contributed equally to this work
Junghwan Oh, email: [email protected]
Jun-O Jin, email: [email protected]
Keywords: lipopolysaccharide; copper sulfide nanoparticles; photothermal therapy; immunotherapy; anti-tumor
Received: August 21, 2017 Accepted: September 22, 2017 Published: November 06, 2017
To meet the ultimate goal of cancer therapy, which is treating not only the primary tumor but also preventing metastatic cancer, the concept of combining immunotherapy with photothermal therapy (PTT) is gaining great interest. Here, we studied the new material, lipopolysaccharide (LPS) coated copper sulfide nanoparticles (LPS-CuS), for the immuno-photothermal therapy. We evaluated the effect of LPS-CuS for induction of apoptosis of CT26 cells and activation of dendritic cells. Moreover, the LPS-CuS and laser irradiation was examined anti-metastasis effect by liver metastasis model mouse in vivo. Through PTT, LPS-CuS induced elimination of CT26 tumor in BALB/c mice, which produced cancer antigens. In addition, released LPS and cancer antigen by PTT promoted dendritic cell activation in tumor draining lymph node (drLN), and consequently, enhanced the tumor antigen-specific immune responses. Finally, the primary tumor cured mice by LPS-CuS-mediated PTT completely resisted secondary tumor injection in the spleen and also prevented liver metastasis. Our results demonstrated the potential usage of LPS-CuS for the immuno-photothermal therapy against various types of cancer by showing the clear elimination of primary colon carcinoma with complete prevention of spleen and liver metastasis.
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