MiR-93-5p enhances growth and angiogenesis capacity of HUVECs by down-regulating EPLIN
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Liang Liang1, Lei Zhao2, Ying Zan1, Qing Zhu1, Juan Ren3 and Xinhan Zhao4
1Department of Oncology, The Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China
2Department of Molecular Physiology and Biophysics, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USA
3Department of Radiation Oncology, The First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061, China
4Department of Oncology, The First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061, China
Xinhan Zhao, email: [email protected]
Juan Ren, email: [email protected]
Keywords: miR-93-5p; EPLIN; angiogenesis; HUVECs; TNBC
Received: March 14, 2017 Accepted: July 12, 2017 Published: November 06, 2017
Tumor angiogenesis is essential in delivering oxygen and nutrients to growing tumors, and therefore considered as a hallmark of cancer. MicroRNAs (miRNAs) have been shown to play important roles in regulating tumor angiogenesis. MicroRNA-93-5p (miR-93-5p) has been identified as an oncogenic miRNA in a variety of human malignancies and involved in tumor angiogenesis in astrocytoma. However, the direct effect(s) of miR-93-5p on the biological behaviors of endothelial cells have not been investigated. Thus, in the present study we investigated the role(s) of miR-93-5p in regulating the functions of human umbilical vein endothelial cells (HUVECs). We found that triple negative breast cancer (TNBC) tissues with higher levels of miR-93-5p showed higher blood vessel density. Overexpression of miR-93-5p accelerated HUVECs proliferation and migration and promoted HUVECs lumen formation and sprouting in vitro, while blockade of miR-93-5p suppressed HUVECs migration and angiogenic capacity. The mechanistic studies revealed that miR-93-5p can promote angiogenic process through inhibiting epithelial protein lost in neoplasm (EPLIN) expression in HUVECs. In sum, our results have indicated that miR-93-5p promoted angiogenesis through down-regulating EPLIN and therefore represented a promising target for developing novel anti-angiogenic therapeutics.
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