Research Papers:

Proteomic analysis of affinity-purified extracellular proteasomes reveals exclusively 20S complexes

Valentina A. Kulichkova, Tatiana O. Artamonova, Olga G. Lyublinskaya, Mikhail A. Khodorkovskii, Alexey N. Tomilin and Anna S. Tsimokha _

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Oncotarget. 2017; 8:102134-102149. https://doi.org/10.18632/oncotarget.22230

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Valentina A. Kulichkova1, Tatiana O. Artamonova2, Olga G. Lyublinskaya1, Mikhail A. Khodorkovskii2, Alexey N. Tomilin1,3 and Anna S. Tsimokha1

1Institute of Cytology, Russian Academy of Sciences, St-Petersburg 194064, Russia

2Institute of Nanobiotechnologies, Peter the Great St-Petersburg Polytechnic University, St-Petersburg 195251, Russia

3Institute of Translational Biomedicine, St-Petersburg State University, St-Petersburg 199034, Russia

Correspondence to:

Anna S. Tsimokha, email: [email protected]

Keywords: extracellular proteasome; human leukemia K562 cells; proteasome interacting protein (PIP); affinity purification; mass spectrometry

Received: April 21, 2017    Accepted: September 29, 2017    Published: November 01, 2017


Proteasome-mediated proteolysis is important for many basic cellular processes. In addition to their functions in the cell, proteasomes have been found in physiological fluids of both healthy and diseased humans including cancer patients. Higher levels of these proteasomes are associated with higher cancer burden and stage. The etiology and functions of these proteasomes, referred to as circulating, plasmatic, or extracellular proteasomes (ex-PSs), are unclear. Here we show that human cancer cell lines, as well as human endometrium-derived mesenchymal stem cells (hMESCs), release proteasome complexes into culture medium (CM). To define ex-PS composition, we have affinity purified them from CM conditioned by human leukemia cell line K562. Using matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS), we have identified core 20S proteasome subunits and a set of 15 proteasome-interacting proteins (PIPs), all previously described as exosome cargo proteins. Three of them, PPIase A, aldolase A, and transferrin, have never been reported as PIPs. The study provides compelling arguments that ex-PSs do not contain 19S or PA200 regulatory particles and are represented exclusively by the 20S complex.

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