Oncotarget

Research Papers:

Transplantation of bFGF-expressing neural stem cells promotes cell migration and functional recovery in rat brain after transient ischemic stroke

Jin-Jing Zhang, Juan-Juan Zhu, Yuan-Bo Hu, Guang-Heng Xiang, Lian-Cheng Deng, Fen-Zan Wu, Xiao-Jie Wei, Ying-Hao Wang, Liang-Yan Sun, Xiao-Qing Lou, Min-Min Shao, Mao Mao, Hong-Yu Zhang, Yue-Ping Xu, Si-Pin Zhu and Jian Xiao _

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Oncotarget. 2017; 8:102067-102077. https://doi.org/10.18632/oncotarget.22155

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Abstract

Jin-Jing Zhang1,4,*, Juan-Juan Zhu3,*, Yuan-Bo Hu2,*, Guang-Heng Xiang2, Lian-Cheng Deng4, Fen-Zan Wu4,5, Xiao-Jie Wei5, Ying-Hao Wang1, Liang-Yan Sun1, Xiao-Qing Lou1, Min-Min Shao1, Mao Mao1, Hong-Yu Zhang4, Yue-Ping Xu1, Si-Pin Zhu2 and Jian Xiao4

1Department of Pharmacy, Affiliated Cixi People’s Hospital, Wenzhou Medical University, Ningbo, Zhejiang, 315300, China

2Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China

3Department of Geriatrics and Neurology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China

4Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

5Department of Neurosurgery, Affiliated Cixi People’s Hospital, Wenzhou Medical University, Ningbo, Zhejiang, 315300, China

*These authors have contributed equally to this work

Correspondence to:

Jian Xiao, email: xfxj2000@126.com

Si-Pin Zhu, email: sipinzhu@163.com

Yue-Ping Xu, email: cxxyp@126.com

Keywords: aging; neural stem cells; basic fibroblast growth factor; ischemic stroke; cell therapy

Received: July 07, 2017     Accepted: October 03, 2017     Published: October 27, 2017

ABSTRACT

Cerebrovascular disease such as stroke is one of the most common diseases in the aging population, and neural stem cells (NSCs) transplantation may provide an alternative therapy for cerebral ischemia. However, a hostile microenvironment in the ischemic brain offers is challenging for the survival of the transplanted cells. Considering the neuroprotective role of basic fibroblast growth factor (bFGF), the present study investigated whether bFGF gene-modified NSCs could improve the neurological function deficit after transient middle cerebral artery occlusion (MCAO) in adult male Sprague–Dawley rats. These rats were intravenously injected with modified NSCs (5×106/200 μL) or vehicle 24 h after MCAO. Histological analysis was performed on days 7 and 28 after tMCAO. The survival, migration, proliferation, and differentiation of the transplanted modified C17.2 cells in the brain were improved. In addition, the intravenous infusion of NSCs and bFGF gene-modified C17.2 cells improved the functional recovery as compared to the control. Furthermore, bFGF promoted the C17.2 cell growth, survival, and differentiation into mature neurons within the infarct region. These data suggested that bFGF gene-modified NSCs have the potential to be a therapeutic agent in brain ischemia.


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