Expression of miR-195 is associated with chemotherapy sensitivity of cisplatin and clinical prognosis in gastric cancer
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Rui Ye1,2,*, Bo Wei3,*, Sheng Li4,*, Wei Liu5,*, Juntao Liu6, Luan Qiu2, Xuan Wu2, Zhifei Zhao2 and Jianxiong Li7
1Department of Oncology, Beidaihe Sanatorium of Beijing Military Command, Qinhuangdao 066100, Hebei, P.R. China
2Department of Radiotherapy, Chinese PLA General Hospital, Beijing 100853, P.R. China
3Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, P.R. China
4Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, P.R. China
5Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100853, P.R. China
6Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, P.R. China
7Department of Radiotherapy, Hainan Branch of Chinese PLA General Hospital, Sanya 572000, Hainan, P.R. China
*These authors have contributed equally to this work
Jianxiong Li, email: [email protected]
Keywords: miR-195; AKT3; gastric cancer; chemotherapy sensitivity
Received: May 27, 2017 Accepted: September 21, 2017 Published: October 19, 2017
Gastric cancer has higher morbidity and mortality than other cancers for the low diagnosis rate and few therapies. MiR-195 has been reported to be involved in the occurrence, development and prognosis of various cancers. However, the function of miR-195 in gastric cancer remains largely unknown. Herein, the aims of this study were to probe the functional mechanism of miR-195 and its chemotherapy sensitivity as well as clinical prognosis in gastric cancer. We screened out low-expressed miR-195 through microarray analysis and further confirmed miR-195 was widely down-regulated in gastric cancer cells. Subsequently, AKT3 was identified as the direct target gene of miR-195 by target gene prediction software, dual luciferase reporter assay and western blot. Functional assays indicated that miR-195 acted as a tumor suppressor through regulating the proliferative, migrated and invasive properties of gastric cancer cells in vitro, and intratumoral delivery of miR-195 significantly suppressed tumor growth in vivo. Additionally, we also found miR-195 overexpression could enhance the chemotherapy sensitivity of cisplatin in gastric cancer cells and prolong the overall survival and progression free survival of gastric cancer patients. Collectively, our findings demonstrate miR-195 may be of great significance on early diagnosis of gastric cancer, providing the theoretical basis for prognosis and recurrence risk.
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