Identifying key regulating miRNAs in hepatocellular carcinomas by an omics' method
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Bing He1,*, Peng Lu2,*, Lei Guan1, Ting Li1, Lei Zhang1, Qing-Ge Zhu1, Xiao-Xiao Ding1, Shi-Ming Zhang1, Xue-Mei Chen1, Jing Zhao1, Song Lin1, Zhi-Zhen Liu1, Fang-E Liu3, Wang Ma4 and Hu-Qin Zhang1
1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, P.R. China
2Gastrointestinal Surgery Department, People’s Hospital of Zhengzhou, Zhengzhou 450052, P.R. China
3Medical College, Xi’an Peihua University, Xi’an 710125, P.R. China
4Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, P.R. China
*These authors have contributed equally to this work
Bing He, email: [email protected]
Fang-E Liu, email: [email protected]
Wang Ma, email: [email protected]
Hu-Qin Zhang, email: [email protected]
Keywords: omics; miRNA; HCC
Received: June 13, 2017 Accepted: September 08, 2017 Published: October 17, 2017
The miRNAs play important regulating roles in the pathogenesis of hepatocellular carcinoma (HCC). To uncover key regulating miRNAs in HCC that were neglected by traditional analyzing methods of transcriptomics data, we proposed a novel molecular-network-based omics’ (MNBO) method. With this method, we predicted HCC-regulating miRNAs, and confirmed the role of a novel miR-590-3P/EED axis by a clinical study and in vitro, in vivo wet-experiments. The miR-590-3P is significantly down-regulated in HCC patients. And low level of miR-590-3P in HCC is associated with poor prognosis of patients. In HCC cell lines, the miR-590-3P suppressed cell proliferation by inhibiting the transformation G1 phase to S phases of the cell cycle. Moreover, the miR-590-3P inhibited migration and invasion of HCC cells. Further investigations indicated that miR-590-3P play its roles by inhibiting polycomb protein EED. The experiments in animal model implied miR-590-3P could be a potential therapeutic agent for HCC in the future. In conclusion, the discovery of miR-590-3P revealed the MNBO would be a useful strategy to uncover key regulating miRNAs in HCC.
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