Research Papers: Immunology:
Effects of baicalein on IL-1β-induced inflammation and apoptosis in rat articular chondrocytes
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Yue Li1, Jinglu Wang1, Xiaopeng Song1, Hui Bai1, Tianwen Ma1, Zhiheng Zhang1, Xinran Li1, Renli Jiang1, Guanying Wang1, Xiaojing Fan1, Xu Liu1 and Li Gao1
1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, People’s Republic of China
Li Gao, email:
Keywords: baicalein, osteoarthritis, chondrocyte, NF-κB, apoptosis
Received: March 03, 2017 Accepted: September 21, 2017 Published: October 11, 2017
In osteoarthritis (OA), activated synoviocytes and articular chondrocytes produce pro-inflammatory cytokines, such as IL-1β, that promote chondrocyte apoptosis and activate the NF-κB signaling pathway to induce catabolic factors. In this study, we examined the anti-inflammatory and anti-apoptotic effect of baicalein on IL-1β signaling and NF-κB-regulated gene products in rat chondrocytes. Rat chondrocytes were pretreated with 10 ng/ml IL-1β for 24 h and then co-treated with 10 ng/ml IL-1β and 50 μM baicalein for 0, 12, 24, 36 and 48h. The expression levels of poly(ADP-ribose) polymerase (PARP), Bcl-2, caspase-3, matrix metalloproteinase (MMP)-9, MMP-3, cyclooxygenase (COX)-2 and SOX-9 were detected by Western blot and quantitative reverse transcription-PCR (qPCR). The effects of baicalein on the translocation and phosphorylation of the NF-κB system were studied by Western blotting and immunofluorescence. Baicalein stimulated the expression of anti-apoptotic genes and reduced the pro-apoptotic and pro-inflammatory gene products in chondrocytes. Baicalein promoted SOX-9 expression in a time-dependent manner in chondrocytes. Baicalein inhibited the NF-κB activation that was induced by IL-1β in a time-dependent manner in chondrocytes. Our results suggest that the anti-inflammatory and anti-apoptotic effects of baicalein are mediated through the inhibition of the translocation of phosphorylated p65 to the nucleus.
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